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Literature summary for 2.5.1.18 extracted from
- Purification and biochemical characterization of cytosolic glutathione-S-transferase from malarial parasites Plasmodium yoelii (2007), Parasitol. Res., 100, 581-588.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hemin | noncompetitive, 86% inhibition at 0.0055 mM | Plasmodium yoelii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
glutathione | pH 6.5, 25°C | Plasmodium yoelii | |
| 3.3 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 25°C | Plasmodium yoelii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Plasmodium yoelii | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Plasmodium yoelii | the soluble enzyme is an essential detoxification enzyme in parasitic species | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium yoelii | - |
subsp. nigeriensis, a rodent malarial parasite, propagated in Swiss albino mice | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 9.9fold by subcellular fractionation, ammonium sulfate fractionation, glutathione affinity chromatography, and ultrafiltration | Plasmodium yoelii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.78 | - |
purified native enzyme | Plasmodium yoelii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme, quite stable | Plasmodium yoelii |
| -80°C, purified enzyme, long term storage, stable | Plasmodium yoelii |
| 4°C, purified enzyme, a few days, complete loss of enzyme activity | Plasmodium yoelii |
| unstable at room temperature | Plasmodium yoelii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Plasmodium yoelii | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
| additional information | the soluble enzyme is an essential detoxification enzyme in parasitic species | Plasmodium yoelii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Plasmodium yoelii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione-S-transferase | - |
Plasmodium yoelii |
| GST | - |
Plasmodium yoelii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Plasmodium yoelii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Plasmodium yoelii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | - |
Plasmodium yoelii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
hemin | pH 6.5, 25°C | Plasmodium yoelii | |
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