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Literature summary for 2.5.1.18 extracted from

  • Griswold, K.E.; Aiyappan, N.S.; Iverson, B.L.; Georgiou, G.
    The evolution of catalytic efficiency and substrate promiscuity in human theta class 1-1 glutathione transferase (2006), J. Mol. Biol., 364, 400-410.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
construction of large libraries of isozyme GST T1-1 constructed by error prone PCR, DNA shuffling, saturation mutagenesis, or a random mutagenesis in conjunction with homologous recombination, screening for improved catalytic activity towards CMAC in a quantitative fashion using flow cytometry, up to a 20000fold increase in kcat/KM compared to wild-type GSTT1-1 is achieved, residue Trp234 is important, overview, recombinant expression of wild-type and mutants as His6-tagged enzymes Homo sapiens
recombinant expression of wild-type and mutants as His6-tagged enzymes Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information chimeric enzymes constructed by homology-independent recombination of human GST T-1 and rat GST T2-2 exhibit very different substrate promiscuity profiles, and show a more defined relationship between evolved and promiscuous activities, overview Rattus norvegicus
additional information chimeric enzymes constructed by homology-independent recombination of human GST T-1 and rat GST T2-2 exhibit very different substrate promiscuity profiles, and show a more defined relationship between evolved and promiscuous activities, overview Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information quantitative screening for improved catalytic activity of mutant isozymes GST T1-1 towards CMAC using flow cytometry, up to a 20000fold increase in kcat/KM compared to wild-type GSTT1-1 is achieved, overview Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P30711 theta class isozyme GST T1-1
-
Rattus norvegicus
-
theta class isozyme GST T2-2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities of wild-type and mutant isozymes GST T1-1, overview Homo sapiens
additional information
-
activities of wild-type and mutant isozymes GST T2-2, overview Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 7-amino-4-chloromethyl coumarin
-
Homo sapiens ?
-
?
glutathione + 7-amino-4-chloromethyl coumarin isozyme GSt T2-2 shows high activity Rattus norvegicus ?
-
?
glutathione + ethacrynic acid
-
Rattus norvegicus ?
-
?
glutathione + ethacrynic acid
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
GST
-
Rattus norvegicus
GST
-
Homo sapiens