Cloned (Comment) | Organism |
---|---|
construction of large libraries of isozyme GST T1-1 constructed by error prone PCR, DNA shuffling, saturation mutagenesis, or a random mutagenesis in conjunction with homologous recombination, screening for improved catalytic activity towards CMAC in a quantitative fashion using flow cytometry, up to a 20000fold increase in kcat/KM compared to wild-type GSTT1-1 is achieved, residue Trp234 is important, overview, recombinant expression of wild-type and mutants as His6-tagged enzymes | Homo sapiens |
recombinant expression of wild-type and mutants as His6-tagged enzymes | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | chimeric enzymes constructed by homology-independent recombination of human GST T-1 and rat GST T2-2 exhibit very different substrate promiscuity profiles, and show a more defined relationship between evolved and promiscuous activities, overview | Rattus norvegicus |
additional information | chimeric enzymes constructed by homology-independent recombination of human GST T-1 and rat GST T2-2 exhibit very different substrate promiscuity profiles, and show a more defined relationship between evolved and promiscuous activities, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | quantitative screening for improved catalytic activity of mutant isozymes GST T1-1 towards CMAC using flow cytometry, up to a 20000fold increase in kcat/KM compared to wild-type GSTT1-1 is achieved, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P30711 | theta class isozyme GST T1-1 | - |
Rattus norvegicus | - |
theta class isozyme GST T2-2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of wild-type and mutant isozymes GST T1-1, overview | Homo sapiens |
additional information | - |
activities of wild-type and mutant isozymes GST T2-2, overview | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 7-amino-4-chloromethyl coumarin | - |
Homo sapiens | ? | - |
? | |
glutathione + 7-amino-4-chloromethyl coumarin | isozyme GSt T2-2 shows high activity | Rattus norvegicus | ? | - |
? | |
glutathione + ethacrynic acid | - |
Rattus norvegicus | ? | - |
? | |
glutathione + ethacrynic acid | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GST | - |
Rattus norvegicus |
GST | - |
Homo sapiens |