Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression analysis | Chlamys islandica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
glutathione | pH 6.5, 24°C | Chlamys islandica | |
0.4 | - |
1-chloro-2,4-dinitrobenzene | pH 6.5, 24°C | Chlamys islandica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
2 * 22000, SDS-PAGE | Chlamys islandica |
44000 | - |
gel filtration | Chlamys islandica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamys islandica | Q0P7I5 | a cold-adapted marine bivalve Iceandic scallop, mu-class enzyme | - |
Purification (Comment) | Organism |
---|---|
native enzyme by glutathione affinity chromatography to homogeneity | Chlamys islandica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
digestive gland | enzyme expression | Chlamys islandica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
300.6 | - |
purified native enzyme | Chlamys islandica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Chlamys islandica | chloride + 2,4-dinitrophenyl-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 22000, SDS-PAGE | Chlamys islandica |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Chlamys islandica |
GST | - |
Chlamys islandica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Chlamys islandica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
55 | maximal activity at 0°C, 10% of maximal activity at 55%, inactive at 60°C | Chlamys islandica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
10 min, loss of 20% of activity | Chlamys islandica |
60 | - |
purified enzyme, rapid complete inactivation | Chlamys islandica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Chlamys islandica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | no activity at pH 4.5, 10% of maximal activity at pH 5.0, 60% at pH 8.0 | Chlamys islandica |