Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3,5,6-tetrachloro-1,4-benzoquinone | i.e. TCBQ, inhibition of GSTA1-2 and GSTA1-1 by 70-80%, and GSTA2-2 by 25%, binds to cysteine residues of the enzyme, e.g. Cys17 of GSTA1-1, via a first reversible step to covalent binding, overview, Kitz-Wilson inactivation model | Rattus norvegicus | |
dinitrophenyl-glutathione | - |
Rattus norvegicus | |
S-hexyl-glutathione | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | alpha class glutathione transferase | Rattus norvegicus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
alpha class isozymes GSTA2-2, GSTA1-2 and GSTA1-1 | - |
Purification (Comment) | Organism |
---|---|
native alpha class isozymes GSTA2-2, GSTA1-2 and GSTA1-1 from kidney by anion exchange and glutathione affinity chromatography, followed by another step of ion exchange chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | mainly alpha class glutathione transferase | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rattus norvegicus | chloride + 2,4-dinitrophenyl-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | homology modeling of recombinant isozyme GSTA1-1 with and without bound inhibitor 2,3,5,6-tetrachloro-1,4-benzoquinone | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
alpha class glutathione transferase | - |
Rattus norvegicus |
GST | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Rattus norvegicus |