Cloned (Comment) | Organism |
---|---|
overexpression of GST adgstD4-4 mutant V107A in Escherichia coli strain BL21(DE3) | Anopheles dirus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant GST adgstD4-4 mutant V107A, hanging drop vapour diffusion method, 0.002 ml of 9 mg/ml protein in 50 mM TrisHCl, pH 7.5, 10 mM DTT, and 10 mM glutathione, are mixed with 0.002 ml of reservoir solution containing 0.1 M imidazole, pH 7.0, 0.35 M ammonium acetate, and 32% PEG 4000, 1 week, 22°C, X-ray diffraction structure determination and analysis at | Anopheles dirus |
Protein Variants | Comment | Organism |
---|---|---|
V107A | site-directed mutagenesis, highly stable enzyme mutant | Anopheles dirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Anopheles dirus | the enzyme is involved in phase II detoxication processes by conjugation of a thiol group from reduced glutathione to an electrophilic centre of diverse xenobiotic compounds, producing less reactive and more polar substances in order to facilitate elimination from cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles dirus | - |
GST adgstD4-4 | - |
Purification (Comment) | Organism |
---|---|
recombinant GST adgstD4-4 mutant V107A from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Anopheles dirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is involved in phase II detoxication processes by conjugation of a thiol group from reduced glutathione to an electrophilic centre of diverse xenobiotic compounds, producing less reactive and more polar substances in order to facilitate elimination from cells | Anopheles dirus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structural model of the wild-type GST adgstD4-4 shows that Val107 is located in the subunit-interface region of the dimeric GST, forming part of an intersubunit lock-and key clasp motif | Anopheles dirus |
Synonyms | Comment | Organism |
---|---|---|
GST adgstD4-4 | - |
Anopheles dirus |