Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pinus tabuliformis |
Protein Variants | Comment | Organism |
---|---|---|
S13A | mutant enzyme shows no significant GST activity with 1-chloro-2,3-dinitrobenzene or with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole | Pinus tabuliformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
1-chloro-2,3-dinitrobenzene | - |
Pinus tabuliformis | |
0.83 | - |
GSH | reaction with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31800 | - |
2 * 31800, SDS-PAGE | Pinus tabuliformis |
60000 | - |
gel filtration | Pinus tabuliformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus tabuliformis | Q6DNI8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pinus tabuliformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
169.1 | - |
- |
Pinus tabuliformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,3-dinitrobenzene + GSH | - |
Pinus tabuliformis | ? | - |
? | |
4-nitrophenyl acetate + GSH | 0.2% of the activity with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis | ? | - |
? | |
7-chloro-4-nitrobenzo-2-oxa-1,3-diazole + GSH | 23.4% of the activity with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis | ? | - |
? | |
ethacrynic acid + GSH | 8% of the activity with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31800, SDS-PAGE | Pinus tabuliformis |
Synonyms | Comment | Organism |
---|---|---|
PtGSTU1 | - |
Pinus tabuliformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 35 | - |
Pinus tabuliformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | more than 60% of maximal activity at 15°C and at 45°C, 50°C: 22% of maximal activity | Pinus tabuliformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
1-chloro-2,3-dinitrobenzene | - |
Pinus tabuliformis | |
6.7 | - |
GSH | reaction with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Pinus tabuliformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: 56% of maximal activity, pH 9.5: 49% of maximal activity, enzyme activity at pH 5.5 and pH 10.5 is nearly abolished | Pinus tabuliformis |