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Literature summary for 2.5.1.18 extracted from

  • Misquitta, S.A.; Colman, R.F.
    Communication between the two active sites of glutathione S-transferase A1-1, probed using wild-type-mutant heterodimers (2005), Biochemistry, 44, 8608-8619.
    View publication on PubMed
Search for more literature for 2.5.1.18

Protein Variants

Protein Variants Comment Organism
additional information construction of heterodimers (WT-Y9F, WT-Y9T, WT-R15Q, WT-R131M, WT-R131Q, and WT-R131E) by incubation of a mixture of wild-type and mutant enzyme at pH 7.5 in buffer containing 1,6-hexanediol, followed by dialysis against buffer lacking the organic solvent. The Vmax values of the resultant heterodimers are lower than expected for independent active sites. Mutation of an amino acid residue in one active site affects the activity in the other active site Rattus norvegicus
R131M mutant enzyme shows 60% of wild-type activity with 1-chloro-2,4-dinitrobenzene as substrate Rattus norvegicus
R131Q mutant enzyme shows 50% of wild-type activity with 1-chloro-2,4-dinitrobenzene as substrate Rattus norvegicus
R131W mutant enzyme shows 24% of wild-type activity with 1-chloro-2,4-dinitrobenzene as substrate Rattus norvegicus
R15Q mutant enzyme shows 0.02% of wild-type activity with 1-chloro-2,4-dinitrobenzene as substrate Rattus norvegicus
Y9F mutant enzyme shows 0.8% of wild-type activity with 1-chloro-2,4-dinitrobenzene as substrate. 4.3fold increase in KM-value for GSH compared to wild-type value, 1.3fold decrease in KM-value for 1-chloro-2,3-dinitrobenzene Rattus norvegicus
Y9T mutant enzyme shows 0.06% of wild-type activity with 1-chloro-2,4-dinitrobenzene as substrate Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 GSH wild-type enzyme Rattus norvegicus
0.63
-
 1-chloro-2,4-dinitrobenzene mutant enzyme Y9F Rattus norvegicus
0.64
-
 1-chloro-2,4-dinitrobenzene mutant enzyme R131M Rattus norvegicus
0.64
-
 1-chloro-2,4-dinitrobenzene mutant enzyme R131Q Rattus norvegicus
0.79
-
 1-chloro-2,4-dinitrobenzene wild-type enzyme Rattus norvegicus
0.8
-
 1-chloro-2,4-dinitrobenzene mutant enzyme R131E Rattus norvegicus
0.82
-
 GSH mutant enzyme R131Q Rattus norvegicus
1
-
 GSH mutant enzyme R131M Rattus norvegicus
1.2
-
 GSH mutant enzyme Y9F Rattus norvegicus
2.1
-
 GSH mutant enzyme R131E Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 recombinant
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 mutant enzyme R15Q Rattus norvegicus
0.035
-
 mutant enzyme Y9T Rattus norvegicus
0.5
-
 mutant enzyme Y9F Rattus norvegicus
14.8
-
 mutant enzyme R131W Rattus norvegicus
30.9
-
 mutant enzyme R131Q Rattus norvegicus
37.1
-
 mutant enzyme R131M Rattus norvegicus
61.5
-
 wild-type enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
 Rattus norvegicus S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Rattus norvegicus

Synonyms

Synonyms Comment Organism
glutathione S-transferase A1-1
-
 Rattus norvegicus