Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Anopheles dirus |
Protein Variants | Comment | Organism |
---|---|---|
E37A | half-life at 45°C is 1.73 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.3fold lower than wild-type value | Anopheles dirus |
E37Q | half-life at 45°C is 6.54 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is nearly identical to wild-type value | Anopheles dirus |
I52A | half-life at 45°C is 9.58 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 100fold lower than wild-type value | Anopheles dirus |
I52L | half-life at 45°C is 18.1 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.4fold lower than wild-type value | Anopheles dirus |
L33A | half-life at 45°C is 45.3 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 2.7fold lower than wild-type value | Anopheles dirus |
L33F | half-life at 45°C is 212 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 40.2fold lower than wild-type value | Anopheles dirus |
L33I | half-life at 45°C is 30.4 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.2fold lower than wild-type value | Anopheles dirus |
L33Y | half-life at 45°C is 71.7 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 186fold lower than wild-type value | Anopheles dirus |
L6A | half-life at 45°C is 1.73 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 1.5fold lower than wild-type value | Anopheles dirus |
T31A | half-life at 45°C is 22.7 min, compared to 15.3 min for wild-type enzyme. Turnover-number for reaction with reduced glutathione and 1-chloro-2,4-dinitrobenzene is 2.7fold lower than wild-type value | Anopheles dirus |
General Stability | Organism |
---|---|
wild-type enzyme becomes completely unfolded after incubation for 10 min in 4 M guanidinium chloride | Anopheles dirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
reduced glutathione | mutant enzyme I52L | Anopheles dirus | |
0.31 | - |
reduced glutathione | mutant enzyme L6A | Anopheles dirus | |
0.32 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme I52A | Anopheles dirus | |
0.42 | - |
reduced glutathione | mutant enzyme L33I | Anopheles dirus | |
0.5 | - |
1-chloro-2,4-dinitrobenzene | wild-type enzyme | Anopheles dirus | |
0.5 | - |
reduced glutathione | wild-type enzyme | Anopheles dirus | |
0.52 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme A35R | Anopheles dirus | |
0.54 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme E37A | Anopheles dirus | |
0.57 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme K42A | Anopheles dirus | |
0.58 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme E37Q | Anopheles dirus | |
0.62 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme K40A | Anopheles dirus | |
0.63 | - |
reduced glutathione | mutant enzyme K42A | Anopheles dirus | |
0.73 | - |
reduced glutathione | mutant enzyme A35R | Anopheles dirus | |
0.73 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L33I | Anopheles dirus | |
0.74 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme I52L | Anopheles dirus | |
0.77 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme T31A | Anopheles dirus | |
0.81 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L33Y | Anopheles dirus | |
1.1 | - |
reduced glutathione | mutant enzyme L33Y | Anopheles dirus | |
1.14 | - |
reduced glutathione | mutant enzyme K40A | Anopheles dirus | |
1.17 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L33A | Anopheles dirus | |
1.3 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L33F | Anopheles dirus | |
1.49 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L6A | Anopheles dirus | |
1.85 | - |
reduced glutathione | mutant enzyme E37A | Anopheles dirus | |
2.26 | - |
reduced glutathione | mutant enzyme E37Q | Anopheles dirus | |
2.42 | - |
reduced glutathione | mutant enzyme L33F | Anopheles dirus | |
7.04 | - |
reduced glutathione | mutant enzyme I52A | Anopheles dirus | |
8.2 | - |
reduced glutathione | mutant enzyme L33A | Anopheles dirus | |
11.4 | - |
reduced glutathione | mutant enzyme T31A | Anopheles dirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE | Anopheles dirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles dirus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Anopheles dirus |
Renatured (Comment) | Organism |
---|---|
wild-type enzyme becomes completely unfolded after incubation for 10 min in 4 M guanidinium chloride. Refolding rate of wild-type enzyme is 0.54 per min, 20.7% recovery | Anopheles dirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced glutathione + 1,2-dichloro-4-nitrobenzene | - |
Anopheles dirus | ? | - |
? | |
reduced glutathione + 1-chloro-2,4-dinitrobenzene | - |
Anopheles dirus | S-2,4-dinitrophenylglutathione + HCl | - |
? | |
reduced glutathione + 4-nitrobenzyl chloride | - |
Anopheles dirus | ? | - |
? | |
reduced glutathione + 4-nitrophenethyl bromide | - |
Anopheles dirus | ? | - |
? | |
reduced glutathione + ethacrynic acid | - |
Anopheles dirus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Anopheles dirus |
Synonyms | Comment | Organism |
---|---|---|
adGSTD4-4 | - |
Anopheles dirus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
half-life: 15.3 min (wild-type enzyme), 45.3 min (mutant enzyme L33A), 71.7 min (mutant enzyme L33Y), 212 min (mutant enzyme L33F), 30.4 min (mutant enzyme L33I), 1.73 min (mutant enzyme L6A), 22.7 min (mutant enzyme T31A), 9.58 min (mutant enzyme I52A), 18.1 min (mutant enzyme I52L), 1.73 min (mutant enzyme L6A), 6.54 min (mutant enzyme E37Q), 3.94 min (mutant enzyme K40A), 8.44 min (mutant enzyme E42A), 9.01 min (mutant enzyme A35R) | Anopheles dirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L33Y | Anopheles dirus | |
0.14 | - |
reduced glutathione | mutant enzyme L33Y | Anopheles dirus | |
0.26 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme I52A | Anopheles dirus | |
0.26 | - |
reduced glutathione | mutant enzyme I52A | Anopheles dirus | |
0.65 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L33F | Anopheles dirus | |
0.65 | - |
reduced glutathione | mutant enzyme L33F | Anopheles dirus | |
9.7 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L33A | Anopheles dirus | |
9.7 | - |
reduced glutathione | mutant enzyme L33A | Anopheles dirus | |
9.73 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme T31A | Anopheles dirus | |
9.73 | - |
reduced glutathione | mutant enzyme T31A | Anopheles dirus | |
17.7 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L6A | Anopheles dirus | |
17.7 | - |
reduced glutathione | mutant enzyme L6A | Anopheles dirus | |
18.3 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme I52L | Anopheles dirus | |
18.3 | - |
reduced glutathione | mutant enzyme I52L | Anopheles dirus | |
20.4 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme E37A | Anopheles dirus | |
20.4 | - |
reduced glutathione | mutant enzyme E37A | Anopheles dirus | |
21.5 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme K40A | Anopheles dirus | |
21.5 | - |
reduced glutathione | mutant enzyme K40A | Anopheles dirus | |
22.2 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme L33I | Anopheles dirus | |
22.2 | - |
reduced glutathione | mutant enzyme L33I | Anopheles dirus | |
22.4 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme A35R | Anopheles dirus | |
22.4 | - |
reduced glutathione | mutant enzyme A35R | Anopheles dirus | |
26.1 | - |
1-chloro-2,4-dinitrobenzene | wild-type enzyme | Anopheles dirus | |
26.1 | - |
reduced glutathione | wild-type enzyme | Anopheles dirus | |
26.3 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme E37Q | Anopheles dirus | |
26.3 | - |
reduced glutathione | mutant enzyme E37Q | Anopheles dirus | |
26.5 | - |
1-chloro-2,4-dinitrobenzene | mutant enzyme K42A | Anopheles dirus | |
26.5 | - |
reduced glutathione | mutant enzyme K42A | Anopheles dirus |