Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.18 extracted from

  • Konishi, T.; Kato, K.; Araki, T.; Shiraki, K.; Takagi, M.; Tamaru, Y.
    A new class of glutathione S-transferase from the hepatopancreas of the red sea bream Pagrus major (2005), Biochem. J., 388, 299-307.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
enzyme form GSTR1 Pagrus major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 GSH enzyme form GSTR1 Pagrus major
0.59
-
 1-chloro-2,4-dinitrobenzene enzyme form GSTR1 Pagrus major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, enzyme form GSTR1, SDS-PAGE Pagrus major
47900
-
 enzyme form GSTR1, gel filtration Pagrus major

Organism

Organism UniProt Comment Textmining
Pagrus major Q50LH1 enzyme form GSTR1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme form GSTR1 Pagrus major

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas enzyme form GSTR1 Pagrus major
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
 Pagrus major S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
additional information no activity with: cumene hydroperoxide, 1,2-dichloro-4-nitrobenzene, ethacrynic acid, 4-hydroxynonenal and p-nitrobenzyl chloride Pagrus major ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, enzyme form GSTR1, SDS-PAGE Pagrus major

Synonyms

Synonyms Comment Organism
pm-GSTR1
-
 Pagrus major

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 enzyme form GSTR1, reaction with 1-chloro-2,4-dinitrobenzene Pagrus major

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 30 20°C: optimum, 30°C: 20% of maximal activity, form GSTR1, reaction with 1-chloro-2,4-dinitrobenzene Pagrus major

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 Tm (melting temperature) is 30.3°C, enzyme form GSTR1 Pagrus major

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.56
-
 1-chloro-2,4-dinitrobenzene enzyme form GSTR1 Pagrus major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 enzyme form GSTR1, reaction with 1-chloro-2,4-dinitrobenzene Pagrus major