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Literature summary for 2.5.1.18 extracted from

  • Friedberg, T.; Milbert, U.; Bentley, P.; Guenther, T.M.; Oesch, F.
    Purification and characterization of a new cytosolic glutathione S-transferase (glutathione S-transferase X) from rat liver (1983), Biochem. J., 215, 617-625.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
2 * 23500, SDS-PAGE Rattus norvegicus
45000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7
-
with 1,2-dichloro-4-nitrobenzene as substrate Rattus norvegicus
21.5
-
with 1-chloro-2,4-dinitrobenzene as substrate Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, pH 7, 10% glycerol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1,2-dichloro-4-nitrobenzene
-
Rattus norvegicus ?
-
?
glutathione + 1,2-epoxy-3-(4-nitrophenoxy)propane
-
Rattus norvegicus ?
-
?
glutathione + 1-chloro-2,4-dinitrobenzene best substrate Rattus norvegicus S-2,4-dinitrophenylglutathione + HCl
-
?
glutathione + 4-nitrobenzyl chloride
-
Rattus norvegicus ?
-
?
glutathione + trans-4-phenyl-3-buten-2-one
-
Rattus norvegicus ?
-
?
additional information specificity Rattus norvegicus ?
-
?
additional information glutathione transferase X: no reaction of menaphthyl sulfate with glutathione, potent in inactivation of tert-10,11-epoxy-r-8,tert-9-dihydroxy-8,9,10,11-tetrahydrobenz/a/anthracene Rattus norvegicus ?
-
?
RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Rattus norvegicus HX + R-S-glutathione
-
?

Subunits

Subunits Comment Organism
dimer 2 * 23500, SDS-PAGE Rattus norvegicus