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Literature summary for 2.5.1.18 extracted from

  • Williamson, G.; Ball, S.K.M.; Chan, H.W.C.
    The purification of multiple forms of glutathione S-transferase from pig liver and their reaction with individual methyl linoleate hydroperoxides (1986), Biochem. Soc. Trans., 14, 1278-1279.
No PubMed abstract available
Search for more literature for 2.5.1.18

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, isoenzyme VII, SDS-PAGE Sus scrofa
26000
-
 1 * 26800 + 1 * 26000, isoenzyme V, SDS-PAGE Sus scrofa
26400
-
 2 * 26400, isoenzyme VI, SDS-PAGE Sus scrofa
26800
-
 1 * 27100 + 1 * 26800, isoenzyme IV, SDS-PAGE Sus scrofa
26800
-
 1 * 26800 + 1 * 26000, isoenzyme V, SDS-PAGE Sus scrofa
27100
-
 1 * 27100 + 1 * 28700, isoenzyme I, SDS-PAGE Sus scrofa
27100
-
 1 * 27100 + 1 * 26800, isoenzyme IV, SDS-PAGE Sus scrofa
28700
-
 1 * 27100 + 1 * 28700, isoenzyme I, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 5 major and 3 minor forms
-

Purification (Commentary)

Purification (Comment) Organism
5 major: I, IV, V, VI, VII and 3 minor forms Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6 11.8 with cumene hydroperoxide as substrate Sus scrofa
7.9 41.1 with 1-chloro-2,4-dinitrobenzene as substrate Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Sus scrofa S-2,4-dinitrophenylglutathione + HCl
-
 ?
glutathione + cumene hydroperoxide
-
 Sus scrofa ?
-
 ?
additional information
-
 Sus scrofa ?
-
 ?
RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Sus scrofa HX + R-S-glutathione
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 26000, isoenzyme VII, SDS-PAGE Sus scrofa
dimer 1 * 27100 + 1 * 28700, isoenzyme I, SDS-PAGE Sus scrofa
dimer 2 * 26400, isoenzyme VI, SDS-PAGE Sus scrofa
dimer 1 * 27100 + 1 * 26800, isoenzyme IV, SDS-PAGE Sus scrofa
dimer 1 * 26800 + 1 * 26000, isoenzyme V, SDS-PAGE Sus scrofa