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Literature summary for 2.5.1.17 extracted from
- Dihydroflavin-driven adenosylation of 4-coordinate Co(II) corrinoids: are cobalamin reductases enzymes or electron transfer proteins? (2010), J. Biol. Chem., 285, 2911-2917.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as an N-terminal His-tagged fusion protein | Homo sapiens |
| expressed in Escherichia coli as an N-terminal His-tagged fusion protein | Limosilactobacillus reuteri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F112A | mutation of the conserved Phe-112 in the active site of PduO is critical for the displacement of cob(II)alamin and leads to an inactive enzyme | Limosilactobacillus reuteri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + cob(I)alamin | Limosilactobacillus reuteri | - |
triphosphate + adenosylcob(III)alamin | adenosyltransferase enzymes lower the thermodynamic barrier of the Co2+ to Co+ reduction needed for the formation of the unique organometalic Co-C bond of adenosylcobalamin | ? |  |
| ATP + cob(I)alamin | Homo sapiens | - |
triphosphate + adenosylcob(III)alamin | adenosyltransferase enzymes lower the thermodynamic barrier of the Co2+ toCo+ reduction needed for the formation of the unique organometalic Co-C bond of adenosylcobalamin | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Limosilactobacillus reuteri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a HisTrap FF column | Homo sapiens |
| using a HisTrap FF column | Limosilactobacillus reuteri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.023 | - |
reductant: PduS protein, specific activity of LrPduO using different electron sources for the adenosylation of cob(II)alamin | Limosilactobacillus reuteri |
| 0.026 | - |
reductant: PduS protein, specific activity of hATR using different electron sources for the adenosylation of cob(II)alamin | Homo sapiens |
| 0.045 | - |
reductant: NADPH-dependent ferredoxin protein reductase, specific activity of LrPduO using different electron sources for the adenosylation of cob(II)alamin | Limosilactobacillus reuteri |
| 0.072 | - |
reductant: NADPH-dependent ferredoxin protein reductase, specific activity of hATR using different electron sources for the adenosylation of cob(II)alamin | Homo sapiens |
| 0.14 | - |
reductant: dihydroflavin, specific activity of LrPduO using different electron sources for the adenosylation of cob(II)alamin | Limosilactobacillus reuteri |
| 0.25 | - |
reductant: dihydroflavin, specific activity of hATR using different electron sources for the adenosylation of cob(II)alamin | Homo sapiens |
| 0.25 | - |
reductant: Ti(III)citrate, specific activity of LrPduO using different electron sources for the adenosylation of cob(II)alamin | Limosilactobacillus reuteri |
| 0.49 | - |
reductant: Ti(III)citrate, specific activity of hATR using different electron sources for the adenosylation of cob(II)alamin | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + cob(I)alamin | - |
Limosilactobacillus reuteri | triphosphate + adenosylcob(III)alamin | - |
? | |
| ATP + cob(I)alamin | - |
Limosilactobacillus reuteri | triphosphate + adenosylcob(III)alamin | adenosyltransferase enzymes lower the thermodynamic barrier of the Co2+ to Co+ reduction needed for the formation of the unique organometalic Co-C bond of adenosylcobalamin | ? | |
| ATP + cob(I)alamin | - |
Homo sapiens | triphosphate + adenosylcob(III)alamin | adenosyltransferase enzymes lower the thermodynamic barrier of the Co2+ toCo+ reduction needed for the formation of the unique organometalic Co-C bond of adenosylcobalamin | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hATR | - |
Homo sapiens |
| LrPduO | - |
Limosilactobacillus reuteri |
| PduO-type corrinoid adenosyltransferase | - |
Homo sapiens |
| PduO-type corrinoid adenosyltransferase | - |
Limosilactobacillus reuteri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Limosilactobacillus reuteri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
| 8 | - |
assay at | Limosilactobacillus reuteri |
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Release 2023.1 (January 2023)
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