Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha as maltose-binding-protein or GST fusion protein | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E193K | site-directed mutagenesis, the mutant enzyme is not expressed | Homo sapiens |
R191W/A135T | site-directed mutagenesis, the mutant enzyme shows 30% reduced activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
0.00037 | - |
cob(I)alamin | pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme | Homo sapiens | |
0.0068 | - |
ATP | pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme | Homo sapiens | |
0.06 | - |
cob(I)alamin | pH 8.0, 37°C, recombinant GST-tagged mutant R191W | Homo sapiens | |
0.32 | - |
ATP | pH 8.0, 37°C, recombinant GST-tagged mutant R191W | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cob(I)alamin + ATP | Homo sapiens | adenosylcobalamin is a cofactor required by the methylmalonyl-CoA mutase | adenosylcobalamin + triphosphate | - |
? | |
additional information | Homo sapiens | enzyme defects cause methylmalonic aciduria type B, regulation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene cblB | - |
Purification (Comment) | Organism |
---|---|
recombinant maltose-binding-protein or GST fusion wild-type and mutant enzymes from Escherichia coli strain DH5alpha by amylose and glutathione affinity chromatography, respectively | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.027 | - |
purified recombinant GST-tagged mutant R191W | Homo sapiens |
0.089 | - |
purified recombinant GST-tagged wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cob(I)alamin + ATP | - |
Homo sapiens | adenosylcobalamin + triphosphate | - |
? | |
cob(I)alamin + ATP | adenosylcobalamin is a cofactor required by the methylmalonyl-CoA mutase | Homo sapiens | adenosylcobalamin + triphosphate | - |
? | |
additional information | enzyme defects cause methylmalonic aciduria type B, regulation, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP:cob(I)alamin adenosyltransferase | - |
Homo sapiens |
MMAB protein | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
cob(I)alamin | pH 8.0, 37°C, recombinant GST-tagged mutant R191W | Homo sapiens | |
0.037 | - |
cob(I)alamin | pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |