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Literature summary for 2.5.1.17 extracted from

  • Zhang, J.; Dobson, C.M.; Wu, X.; Lerner-Ellis, J.; Rosenblatt, D.S.; Gravel, R.A.
    Impact of cblB mutations on the function of ATP:cob(I)alamin adenosyltransferase in disorders of vitamin B12 metabolism (2006), Mol. Genet. Metab., 87, 315-322.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha as maltose-binding-protein or GST fusion protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
E193K site-directed mutagenesis, the mutant enzyme is not expressed Homo sapiens
R191W/A135T site-directed mutagenesis, the mutant enzyme shows 30% reduced activity compared to the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics Homo sapiens
0.00037
-
cob(I)alamin pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme Homo sapiens
0.0068
-
ATP pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme Homo sapiens
0.06
-
cob(I)alamin pH 8.0, 37°C, recombinant GST-tagged mutant R191W Homo sapiens
0.32
-
ATP pH 8.0, 37°C, recombinant GST-tagged mutant R191W Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cob(I)alamin + ATP Homo sapiens adenosylcobalamin is a cofactor required by the methylmalonyl-CoA mutase adenosylcobalamin + triphosphate
-
?
additional information Homo sapiens enzyme defects cause methylmalonic aciduria type B, regulation, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gene cblB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose-binding-protein or GST fusion wild-type and mutant enzymes from Escherichia coli strain DH5alpha by amylose and glutathione affinity chromatography, respectively Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.027
-
purified recombinant GST-tagged mutant R191W Homo sapiens
0.089
-
purified recombinant GST-tagged wild-type enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cob(I)alamin + ATP
-
Homo sapiens adenosylcobalamin + triphosphate
-
?
cob(I)alamin + ATP adenosylcobalamin is a cofactor required by the methylmalonyl-CoA mutase Homo sapiens adenosylcobalamin + triphosphate
-
?
additional information enzyme defects cause methylmalonic aciduria type B, regulation, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
ATP:cob(I)alamin adenosyltransferase
-
Homo sapiens
MMAB protein
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.011
-
cob(I)alamin pH 8.0, 37°C, recombinant GST-tagged mutant R191W Homo sapiens
0.037
-
cob(I)alamin pH 8.0, 37°C, recombinant wild-type GST-tagged enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens