Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, expression of 2 polymorphic variants 239K and 239M of the enzyme in Escherichia coli as soluble proteins | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens | |
0.0012 | - |
cob(I)alamin | recombinant enzyme variant 239K | Homo sapiens | |
0.0016 | - |
cob(I)alamin | recombinant enzyme variant 239M | Homo sapiens | |
0.0063 | - |
ATP | recombinant enzyme variant 239K | Homo sapiens | |
0.0069 | - |
ATP | recombinant enzyme variant 239M | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | enzyme contains a putative mitochondrial targeting sequence | Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity decreases in absence of Mg2+ by 20% | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | Homo sapiens | polymorphic variants 239K and 239M, biosynthesis of adenosylcobalamin | triphosphate + adenosylcobalamin | - |
? | |
additional information | Homo sapiens | the enzyme interacts with the methionine synthase reductase MSR, which catalyzes the reduction of cob(II)almin to cob(I)alamin, both enzymes activate each other, stoichiometry of the MSR-ATR system, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
2 common polymorphic variants 239K and 239M of the enzyme | - |
Purification (Comment) | Organism |
---|---|
2 recombinant polymorphic variants 239K and 239M of the enzyme from Escherichia coli in a multistep procedure to homogeneity, 4.5- and 5.4fold, respectively | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.19 | - |
recombinant enzyme variant 239M | Homo sapiens |
0.22 | - |
recombinant enzyme variant 239K | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | polymorphic variants 239K and 239M, biosynthesis of adenosylcobalamin | Homo sapiens | triphosphate + adenosylcobalamin | - |
? | |
ATP + cob(I)alamin | polymorphic variants 239K and 239M, ATP is highly preferred as adenosyl donor | Homo sapiens | triphosphate + adenosylcobalamin | - |
? | |
CTP + cob(I)alamin | polymorphic variants 239K and 239M, 9% activity compared to ATP with enzyme variant 239K, 6% activity compared to ATP with enzyme variant 239M | Homo sapiens | triphosphate + cytosylcobalamin | - |
? | |
GTP + cob(I)alamin | polymorphic variants 239K and 239M, 16% activity compared to ATP with enzyme variant 239K, 14% activity compared to ATP with enzyme variant 239M | Homo sapiens | triphosphate + guanosylcobalamin | - |
? | |
additional information | the enzyme interacts with the methionine synthase reductase MSR, which catalyzes the reduction of cob(II)almin to cob(I)alamin, both enzymes activate each other, stoichiometry of the MSR-ATR system, overview | Homo sapiens | ? | - |
? | |
additional information | no activity with ADP and AMP | Homo sapiens | ? | - |
? | |
UTP + cob(I)alamin | polymorphic variants 239K and 239M, 8% activity compared to ATP with enzyme variant 239K, 6% activity compared to ATP with enzyme variant 239M | Homo sapiens | triphosphate + uridylcobalamin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP:cob(I)alamin adenosyltransferase | - |
Homo sapiens |
ATR | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |