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Literature summary for 2.5.1.17 extracted from

  • Leal, N.A.; Olteanu, H.; Banerjee, R.; Bobik, T.A.
    Human ATP:cob(I)alamin adenosyltransferase and its interaction with methionine synthase reductase (2004), J. Biol. Chem., 279, 47536-47542.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, expression of 2 polymorphic variants 239K and 239M of the enzyme in Escherichia coli as soluble proteins Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens
0.0012
-
cob(I)alamin recombinant enzyme variant 239K Homo sapiens
0.0016
-
cob(I)alamin recombinant enzyme variant 239M Homo sapiens
0.0063
-
ATP recombinant enzyme variant 239K Homo sapiens
0.0069
-
ATP recombinant enzyme variant 239M Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion enzyme contains a putative mitochondrial targeting sequence Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity decreases in absence of Mg2+ by 20% Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cob(I)alamin Homo sapiens polymorphic variants 239K and 239M, biosynthesis of adenosylcobalamin triphosphate + adenosylcobalamin
-
?
additional information Homo sapiens the enzyme interacts with the methionine synthase reductase MSR, which catalyzes the reduction of cob(II)almin to cob(I)alamin, both enzymes activate each other, stoichiometry of the MSR-ATR system, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
2 common polymorphic variants 239K and 239M of the enzyme
-

Purification (Commentary)

Purification (Comment) Organism
2 recombinant polymorphic variants 239K and 239M of the enzyme from Escherichia coli in a multistep procedure to homogeneity, 4.5- and 5.4fold, respectively Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.19
-
recombinant enzyme variant 239M Homo sapiens
0.22
-
recombinant enzyme variant 239K Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamin polymorphic variants 239K and 239M, biosynthesis of adenosylcobalamin Homo sapiens triphosphate + adenosylcobalamin
-
?
ATP + cob(I)alamin polymorphic variants 239K and 239M, ATP is highly preferred as adenosyl donor Homo sapiens triphosphate + adenosylcobalamin
-
?
CTP + cob(I)alamin polymorphic variants 239K and 239M, 9% activity compared to ATP with enzyme variant 239K, 6% activity compared to ATP with enzyme variant 239M Homo sapiens triphosphate + cytosylcobalamin
-
?
GTP + cob(I)alamin polymorphic variants 239K and 239M, 16% activity compared to ATP with enzyme variant 239K, 14% activity compared to ATP with enzyme variant 239M Homo sapiens triphosphate + guanosylcobalamin
-
?
additional information the enzyme interacts with the methionine synthase reductase MSR, which catalyzes the reduction of cob(II)almin to cob(I)alamin, both enzymes activate each other, stoichiometry of the MSR-ATR system, overview Homo sapiens ?
-
?
additional information no activity with ADP and AMP Homo sapiens ?
-
?
UTP + cob(I)alamin polymorphic variants 239K and 239M, 8% activity compared to ATP with enzyme variant 239K, 6% activity compared to ATP with enzyme variant 239M Homo sapiens triphosphate + uridylcobalamin
-
?

Synonyms

Synonyms Comment Organism
ATP:cob(I)alamin adenosyltransferase
-
Homo sapiens
ATR
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens