Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme in Escherichia coli in inclusion bodies, expression of truncated mutants in Escherichia coli | Salmonella enterica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several truncated mutants, the activity decreases with truncation degree, remaining residues 1-90 are inactive, overview | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxyadenosine | weak inhibition | Salmonella enterica | |
adenine | weak inhibition | Salmonella enterica | |
adenosine | weak inhibition | Salmonella enterica | |
ADP | about 90% inhibition | Salmonella enterica | |
Cd2+ | complete inhibition | Salmonella enterica | |
Cu2+ | complete inhibition | Salmonella enterica | |
additional information | CTP, GTP, UTP, and AMP are no inhibitors | Salmonella enterica | |
Ni2+ | complete inhibition | Salmonella enterica | |
Zn2+ | complete inhibition | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Salmonella enterica | |
0.0045 | - |
cob(I)alamin | pH 8.0, 37°C, recombinant full length wild-type enzyme | Salmonella enterica | |
0.0198 | - |
ATP | pH 8.0, 37°C, recombinant full length wild-type enzyme | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activity with divalent cations in descending order: Mg2+, Mn2+, Co2+ | Salmonella enterica | |
Mg2+ | required for activity, in absence of Mg2+ activity is reduced to 32% of maximum activity, activity with divalent cations in descending order: Mg2+, Mn2+, Co2+ | Salmonella enterica | |
Mn2+ | activity with divalent cations in descending order: Mg2+, Mn2+, Co2+ | Salmonella enterica | |
additional information | Ca2+ has no effect on enzyme activity | Salmonella enterica | |
titanium citrate | - |
Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36812 | - |
x * 36812, sequence calculation, full length enzyme | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | Salmonella enterica | final step in the conversion of vitamin B12 to coenzyme B12, the latter being required for degradation of 1,2-propanediol | triphosphate + adenosylcob(III)alamin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant solubilized enzyme from Escherichia coli 1.4fold to homogeneity, partial purification of recombinant truncated mutants, recombinant N-terminal domain to homogeneity | Salmonella enterica |
Renatured (Comment) | Organism |
---|---|
solubilization of wild-type enzyme and truncated enzyme forms from inclusion bodies after expression in Escherichia coli | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of recombinant truncated enzyme versions | Salmonella enterica |
0.091 | - |
purified recombinant full length wild-type enzyme, in absence of Mg2+ | Salmonella enterica |
0.262 | - |
purified recombinant full length wild-type enzyme, in presence of 2.5 mM MgCl2 | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | final step in the conversion of vitamin B12 to coenzyme B12, the latter being required for degradation of 1,2-propanediol | Salmonella enterica | triphosphate + adenosylcob(III)alamin | - |
? | |
ATP + cobalamin | enzyme is absolutely specific for ATP or dATP as adenosyl donors, ATP is the preferred adenosyl donor | Salmonella enterica | triphosphate + adenosylcob(III)alamin | - |
? | |
dATP + cobalamin | enzyme is absolutely specific for ATP or dATP as adenosyl donors, dATP results in 21% of the activity with ATP | Salmonella enterica | triphosphate + deoxyadenosylcob(III)alamin | - |
? | |
additional information | random or ordered ternary complex mechanism, the N-terminal domain is responsible for the catalytic activity and substrate binding and has similar biochemical properties and kinetic constants as the full-length enzyme | Salmonella enterica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36812, sequence calculation, full length enzyme | Salmonella enterica |
More | approximate molecular weight of full length and truncated mutants | Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
ATP:cob(I)alamin adenosyltransferase | - |
Salmonella enterica |
PduO | - |
Salmonella enterica |
PduO adenosyltransferase | - |
Salmonella enterica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | recombinant truncated enzyme versions | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Salmonella enterica |