Any feedback?
Literature summary for 2.5.1.17 extracted from
- The eutT gene of Salmonella enterica encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme (2004), J. Bacteriol., 186, 5708-5714.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme B12 | i.e. adenosylcob(III)alamin, is required for stimulation of eut operon expression | Salmonella enterica |  |
| coenzyme B12 | i.e. adenosylcob(III)alamin, is required for stimulation of eut operon expression | Salmonella enterica subsp. enterica serovar Typhimurium | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview | Salmonella enterica |
| 2 enzymes encoded by genes eutT and cobA, DNA sequence determination, analysis of several geno- and phenotypic strains, enzyme is expressed e.g. as fusion protein or to complement a deficient strain, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bathophenanthroline | inhibition of EutT | Salmonella enterica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required and contained by EutT | Salmonella enterica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + cob(I)alamin | Salmonella enterica | last step in the corrinoid adenosylation pathway, EutT and CobA | triphosphate + adenosylcob(III)alamin | - |
ir | |
| ATP + cob(I)alamin | Salmonella enterica subsp. enterica serovar Typhimurium | last step in the corrinoid adenosylation pathway, EutT and CobA | triphosphate + adenosylcob(III)alamin | - |
ir | |
| additional information | Salmonella enterica | EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source | ? | - |
? | |
| additional information | Salmonella enterica subsp. enterica serovar Typhimurium | EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | - |
EutT; 2 enzymes encoded by genes eutT and cobA with different primary sequences, cobA is a housekeeping gene, several derivatives of strain TR6583, several geno- and phenotypes, overview | - |
| Salmonella enterica subsp. enterica serovar Typhimurium | P31570 | CobA; 2 enzymes encoded by genes eutT and cobA with different primary sequences, cobA is a housekeeping gene, several derivatives of strain TR6583, several geno- and phenotypes, overview | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| EutT is oxygen-labile, exposure to air leads to complete loss of activity | Salmonella enterica |
| EutT is oxygen-labile, exposure to air leads to complete loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0061 | - |
strain carrying the complementation plasmd eutT+, in vivo activity | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.0061 | - |
strain carrying the complementation plasmid eutT+, in vivo activity | Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + cob(I)alamin | last step in the corrinoid adenosylation pathway, EutT and CobA | Salmonella enterica | triphosphate + adenosylcob(III)alamin | - |
ir | |
| ATP + cob(I)alamin | last step in the corrinoid adenosylation pathway, EutT and CobA | Salmonella enterica subsp. enterica serovar Typhimurium | triphosphate + adenosylcob(III)alamin | - |
ir | |
| ATP + cob(I)alamin | CobA has an ATP-binding P-loop motif, while EutT has a cysteine-rich region reminiscent of a conserved S-adenosylmethionine Fe-S cluster motif | Salmonella enterica | triphosphate + adenosylcob(III)alamin | - |
ir | |
| ATP + cob(I)alamin | CobA has an ATP-binding P-loop motif, while EutT has a cysteine-rich region reminiscent of a conserved S-adenosylmethionine Fe-S cluster motif | Salmonella enterica subsp. enterica serovar Typhimurium | triphosphate + adenosylcob(III)alamin | - |
ir | |
| additional information | EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source | Salmonella enterica | ? | - |
? | |
| additional information | EutT is necessary and sufficient for growth of a cobA eutT strain on ethanolamine as a carbon and energy or nitrogen source | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP:co(I)rrinoid adenosyltransferase | - |
Salmonella enterica |
| ATP:co(I)rrinoid adenosyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| ATP:corrinoid adenosyltransferase | - |
Salmonella enterica |
| ATP:corrinoid adenosyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| CobA | - |
Salmonella enterica |
| CobA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| cobalamin adenosyltransferase | - |
Salmonella enterica |
| cobalamin adenosyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| EutT | - |
Salmonella enterica |
| EutT | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Salmonella enterica |
| 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Salmonella enterica |
| 8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
