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Literature summary for 2.5.1.17 extracted from

  • Debussche, L.; Couder, M.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F.
    Purification and partial characterization of Cob(I)alamin adenosyltransferase from Pseudomonas denitrificans (1991), J. Bacteriol., 173, 6300-6302.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
DTT stabilizes during purification and storage, removal leads to complete loss of activity Pseudomonas denitrificans (nom. rej.)

Inhibitors

Inhibitors Comment Organism Structure
hydrogenobyrinic acid a,c-diamide cobalt-free analogue of cobyrinic acid a,c-diamide Pseudomonas denitrificans (nom. rej.)
additional information no inhibition by cobyrinic acid, hydrogenobyrinic acid Pseudomonas denitrificans (nom. rej.)
tripolyphosphate
-
Pseudomonas denitrificans (nom. rej.)

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Pseudomonas denitrificans (nom. rej.)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
2 * 28000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
56000
-
HPLC gel filtration Pseudomonas denitrificans (nom. rej.)
70000
-
PAGE Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cob(I)alamin Pseudomonas denitrificans (nom. rej.)
-
triphosphate + coenzyme B12
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.) P29930 recombinant strain SC510 Rifr containing plasmid pXL227, this DNA seuqence encodes for several enzymes
-

Purification (Commentary)

Purification (Comment) Organism
centrifugation, Mono Q chromatography and gel filtration Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.089
-
pH 8.0, 30°C Pseudomonas denitrificans (nom. rej.)
0.089
-
in the presence of cobalamin as substrate Pseudomonas denitrificans (nom. rej.)
0.13
-
pH 8.0, 30°C Pseudomonas denitrificans (nom. rej.)
0.13
-
in the presence of cobyrinic acid a,c-diamide as substrate Pseudomonas denitrificans (nom. rej.)
0.14
-
pH 8.0, 30°C Pseudomonas denitrificans (nom. rej.)
0.14
-
in the presence of cobyric acid as substrate Pseudomonas denitrificans (nom. rej.)
0.18
-
pH 8.0, 30°C Pseudomonas denitrificans (nom. rej.)
0.18
-
in the presence of cobinamide as substrate Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamin
-
Pseudomonas denitrificans (nom. rej.) triphosphate + coenzyme B12
-
?
ATP + cob(I)alamin
-
Pseudomonas denitrificans (nom. rej.) triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
cob(I)inamide + ATP
-
Pseudomonas denitrificans (nom. rej.) 5'-deoxy-5'-adenosyl-cob(I)inamide + polyphosphate
-
?
cob(I)yric acid + ATP
-
Pseudomonas denitrificans (nom. rej.) 5'-deoxy-5'-adenosyl-cob(I)yric acid + polyphosphate
-
?
cob(I)yrinic acid a,c-diamide + ATP
-
Pseudomonas denitrificans (nom. rej.) 5'-deoxy-5'-adenosyl-cob(I)yrinic acid a,c-diamide + polyphosphate
-
?
additional information cobyrinic acid is not a substrate Pseudomonas denitrificans (nom. rej.) ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas denitrificans (nom. rej.)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pseudomonas denitrificans (nom. rej.)