General Stability | Organism |
---|---|
DTT stabilizes during purification and storage, removal leads to complete loss of activity | Pseudomonas denitrificans (nom. rej.) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydrogenobyrinic acid a,c-diamide | cobalt-free analogue of cobyrinic acid a,c-diamide | Pseudomonas denitrificans (nom. rej.) | |
additional information | no inhibition by cobyrinic acid, hydrogenobyrinic acid | Pseudomonas denitrificans (nom. rej.) | |
tripolyphosphate | - |
Pseudomonas denitrificans (nom. rej.) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pseudomonas denitrificans (nom. rej.) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Pseudomonas denitrificans (nom. rej.) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
56000 | - |
HPLC gel filtration | Pseudomonas denitrificans (nom. rej.) |
70000 | - |
PAGE | Pseudomonas denitrificans (nom. rej.) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | Pseudomonas denitrificans (nom. rej.) | - |
triphosphate + coenzyme B12 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas denitrificans (nom. rej.) | P29930 | recombinant strain SC510 Rifr containing plasmid pXL227, this DNA seuqence encodes for several enzymes | - |
Purification (Comment) | Organism |
---|---|
centrifugation, Mono Q chromatography and gel filtration | Pseudomonas denitrificans (nom. rej.) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.089 | - |
pH 8.0, 30°C | Pseudomonas denitrificans (nom. rej.) |
0.089 | - |
in the presence of cobalamin as substrate | Pseudomonas denitrificans (nom. rej.) |
0.13 | - |
pH 8.0, 30°C | Pseudomonas denitrificans (nom. rej.) |
0.13 | - |
in the presence of cobyrinic acid a,c-diamide as substrate | Pseudomonas denitrificans (nom. rej.) |
0.14 | - |
pH 8.0, 30°C | Pseudomonas denitrificans (nom. rej.) |
0.14 | - |
in the presence of cobyric acid as substrate | Pseudomonas denitrificans (nom. rej.) |
0.18 | - |
pH 8.0, 30°C | Pseudomonas denitrificans (nom. rej.) |
0.18 | - |
in the presence of cobinamide as substrate | Pseudomonas denitrificans (nom. rej.) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | - |
Pseudomonas denitrificans (nom. rej.) | triphosphate + coenzyme B12 | - |
? | |
ATP + cob(I)alamin | - |
Pseudomonas denitrificans (nom. rej.) | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
cob(I)inamide + ATP | - |
Pseudomonas denitrificans (nom. rej.) | 5'-deoxy-5'-adenosyl-cob(I)inamide + polyphosphate | - |
? | |
cob(I)yric acid + ATP | - |
Pseudomonas denitrificans (nom. rej.) | 5'-deoxy-5'-adenosyl-cob(I)yric acid + polyphosphate | - |
? | |
cob(I)yrinic acid a,c-diamide + ATP | - |
Pseudomonas denitrificans (nom. rej.) | 5'-deoxy-5'-adenosyl-cob(I)yrinic acid a,c-diamide + polyphosphate | - |
? | |
additional information | cobyrinic acid is not a substrate | Pseudomonas denitrificans (nom. rej.) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas denitrificans (nom. rej.) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas denitrificans (nom. rej.) |