Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
in complex with decarboxylated S-adenosylmethionine, trans-4-methylcyclohexylamine and inhibitors, sitting drop vapor diffusion method, using 100 mM bis-Tris pH 5.5-6.5, 200 mM ammonium sulfate, 10-15% (w/v) PEG 4000 | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-phenyl-1,2-thiazol-3(2H)-one | - |
Trypanosoma cruzi | |
2H-1,4-benzothiazin-3-amine | - |
Trypanosoma cruzi | |
5-[(4-methylbenzyl)oxy]quinazolin-2-amine | - |
Trypanosoma cruzi | |
isoquinolin-1-amine | - |
Trypanosoma cruzi | |
N-methyl-N-phenyl-2,3-dihydro-1,4-benzodioxine-2-carboxamide | - |
Trypanosoma cruzi | |
quinolin-8-yl piperidine-1-carboxylate | - |
Trypanosoma cruzi | |
S-adenosyl-1,8-diamino-3-thio-octane | - |
Trypanosoma cruzi | |
trans-4-Methylcyclohexylamine | - |
Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl 3-(methylthio)propylamine + putrescine | Trypanosoma cruzi | - |
S-methyl-5'-thioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4DA73 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 75 gel filtration | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl 3-(methylthio)propylamine + putrescine | - |
Trypanosoma cruzi | S-methyl-5'-thioadenosine + spermidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
SPDSYN | - |
Trypanosoma cruzi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000051 | - |
pH and temperature not specified in the publication | Trypanosoma cruzi | 2-phenyl-1,2-thiazol-3(2H)-one | |
0.0091 | - |
pH and temperature not specified in the publication | Trypanosoma cruzi | 5-[(4-methylbenzyl)oxy]quinazolin-2-amine | |
0.18 | - |
pH and temperature not specified in the publication | Trypanosoma cruzi | 2H-1,4-benzothiazin-3-amine | |
0.46 | - |
pH and temperature not specified in the publication | Trypanosoma cruzi | isoquinolin-1-amine | |
0.53 | - |
pH and temperature not specified in the publication | Trypanosoma cruzi | N-methyl-N-phenyl-2,3-dihydro-1,4-benzodioxine-2-carboxamide | |
0.83 | - |
pH and temperature not specified in the publication | Trypanosoma cruzi | quinolin-8-yl piperidine-1-carboxylate |