Literature summary for 2.5.1.16 extracted from

Lu, P.K.; Tsai, J.Y.; Chien, H.Y.; Huang, H.; Chu, C.H.; Sun, Y.J.
Crystal structure of Helicobacter pylori spermidine synthase: a Rossmann-like fold with a distinct active site (2007), Proteins, 67, 743-754.

Cloned(Commentary)

Cloned (Comment)	Organism
gene speE, sequence comparison, overexpression of the N-terminally His6-tagged enzyme in Escherichia coli strain SG13009 and in strain B834 for the selenomethionine-labeled enzyme	Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His6-tagged enzyme, also as selenomethionine-labeled enzyme, the latter from 0.1 M sodium HEPES, pH 7.5, with 33% PEG 400, two crystals Forms A and B, belonging to the monoclinic space group P21and orthorhombic space group C2221, are grown under different conditions, X-ray diffraction structure determination and analysis at 2.0-2.5 A resolution, multiwavelength anomalous dispersion	Helicobacter pylori

Crystallization (Comment)

Organism

purified recombinant His6-tagged enzyme, also as selenomethionine-labeled enzyme, the latter from 0.1 M sodium HEPES, pH 7.5, with 33% PEG 400, two crystals Forms A and B, belonging to the monoclinic space group P21and orthorhombic space group C2221, are grown under different conditions, X-ray diffraction structure determination and analysis at 2.0-2.5 A resolution, multiwavelength anomalous dispersion

Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	dimeric enzyme form A, analytical ultracentrifugation	Helicobacter pylori
60000	-	dimeric enzyme form A, gel filtration	Helicobacter pylori
123000	-	tetrameric enzyme form B, analytical ultracentrifugation	Helicobacter pylori
158000	-	tetrameric enzyme form B, gel filtration	Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosylmethioninamine + putrescine	Helicobacter pylori	PAPTs are essential for bacterial cell viability	5'-S-methyl-5'-thioadenosine + spermidine	-	?

Organism

Organism	UniProt	Comment	Textmining
Helicobacter pylori	-	gene speE, strain 26695	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography	Helicobacter pylori

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine	the enzyme possesses a Rossmann-like fold with a distinct active site, the enzyme from Helicobacter pylori lacks the gatekeeping loop that facilitates substrate binding in other PAPTs, active site structure	Helicobacter pylori	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosylmethioninamine + putrescine	-	Helicobacter pylori	5'-S-methyl-5'-thioadenosine + spermidine	-	?
S-adenosylmethioninamine + putrescine	PAPTs are essential for bacterial cell viability	Helicobacter pylori	5'-S-methyl-5'-thioadenosine + spermidine	-	?

Subunits

Subunits	Comment	Organism
dimer	dimer interaction in enzyme form A crystals, overview, the enzyme shows an N-terminal beta-stranded domain and a C-terminal Rossmann-like domain, structure comparison	Helicobacter pylori
tetramer	dimer of dimer assembly, enzyme form B crystals, overview, the enzyme shows an N-terminal beta-stranded domain and a C-terminal Rossmann-like domain, structure comparison	Helicobacter pylori

Synonyms

Synonyms	Comment	Organism
PAPT	-	Helicobacter pylori
putrescine aminopropyltransferase	-	Helicobacter pylori