Cloned (Comment) | Organism |
---|---|
overexpression of soluble FLAG-tagged enzyme in HEK-293T cell cytoplasm | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-methylcyclohexylamine | - |
Homo sapiens | |
S-adenosyl-L-methionine | above 0.01 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosylmethioninamine + putrescine | Homo sapiens | - |
5'-S-methyl-5'-thioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged enzyme from HEK-293 cells by affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
development of a high-throughput assay using homogeneous time-resolved fluorescence based on energy transfer from europium cryptate as a donor to crosslinked allophycocyanin XL665 as an acceptor, involving a competitive immunoassay, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosylmethioninamine + putrescine | - |
Homo sapiens | 5'-S-methyl-5'-thioadenosine + spermidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000-40000, recombinant FLAG-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
SPDS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |