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Literature summary for 2.5.1.16 extracted from

  • Enomoto, K.; Nagasaki, T.; Yamauchi, A.; Onoda, J.; Sakai, K.; Yoshida, T.; Maekawa, K.; Kinoshita, Y.; Nishino, I.; Kikuoka, S.; Fukunaga, T.; Kawamoto, K.; Numata, Y.; Takemoto, H.; Nagata, K.
    Development of high-throughput spermidine synthase activity assay using homogeneous time-resolved fluorescence (2006), Anal. Biochem., 351, 229-240.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of soluble FLAG-tagged enzyme in HEK-293T cell cytoplasm Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-methylcyclohexylamine
-
Homo sapiens
S-adenosyl-L-methionine above 0.01 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosylmethioninamine + putrescine Homo sapiens
-
5'-S-methyl-5'-thioadenosine + spermidine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged enzyme from HEK-293 cells by affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
development of a high-throughput assay using homogeneous time-resolved fluorescence based on energy transfer from europium cryptate as a donor to crosslinked allophycocyanin XL665 as an acceptor, involving a competitive immunoassay, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosylmethioninamine + putrescine
-
Homo sapiens 5'-S-methyl-5'-thioadenosine + spermidine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000-40000, recombinant FLAG-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
SPDS
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens