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Literature summary for 2.5.1.126 extracted from

  • Knott, J.M.
    Biosynthesis of long-chain polyamines by crenarchaeal polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum (2009), FEBS Lett., 583, 3519-3524.
    View publication on PubMed
Search for more literature for 2.5.1.126

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with an N-terminal 6His sequence Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32800
-
 x * 32800, calculation from sequence Pyrobaculum aerophilum
37000
-
 x * 37000, SDS-PAGE Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine Pyrobaculum aerophilum DSM 7523 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine Pyrobaculum aerophilum DSM 7523 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + norspermine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldopentamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine Pyrobaculum aerophilum the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + norspermidine
-
 ?
S-adenosyl 3-(methylthio)propylamine + spermidine Pyrobaculum aerophilum spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines S-methyl-5'-thioadenosine + thermospermine + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum Q8ZXM4
-
-
Pyrobaculum aerophilum DSM 7523 Q8ZXM4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
 pH 7.5, 95°C, substrate: norspermine Pyrobaculum aerophilum
0.052
-
 pH 7.5, 95°C, substrate: spermidine Pyrobaculum aerophilum
0.09
-
 pH 7.5, 95°C, substrate: propane-1,3-diamine Pyrobaculum aerophilum
0.14
-
 pH 7.5, 95°C, substrate: norspermidine Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
 Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
 Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
 Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
 Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine
-
 Pyrobaculum aerophilum S-methyl-5'-thioadenosine + norspermine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + norspermine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine
-
 Pyrobaculum aerophilum DSM 7523 S-methyl-5'-thioadenosine + norspermine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldopentamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermine at 4% of the specific activity compared to norspermidine Pyrobaculum aerophilum S-methyl-5'-thioadenosine + caldopentamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Pyrobaculum aerophilum S-methyl-5'-thioadenosine + norspermidine
-
 ?
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine at 64% of the specific activity compared to norspermidine Pyrobaculum aerophilum S-methyl-5'-thioadenosine + norspermidine
-
 ?
S-adenosyl 3-(methylthio)propylamine + spermidine spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines Pyrobaculum aerophilum S-methyl-5'-thioadenosine + thermospermine + H+
-
 ?
S-adenosyl 3-(methylthio)propylamine + spermidine at 37% of the specific activity compared to norspermidine Pyrobaculum aerophilum S-methyl-5'-thioadenosine + thermospermine + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Pyrobaculum aerophilum
? x * 32800, calculation from sequence Pyrobaculum aerophilum

Synonyms

Synonyms Comment Organism
long-chain polyamine synthase ambiguous Pyrobaculum aerophilum
PaSpeE
-
 Pyrobaculum aerophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 assay at Pyrobaculum aerophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pyrobaculum aerophilum