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Literature summary for 2.5.1.123 extracted from
- A soluble, magnesium-independent prenyltransferase catalyzes reverse and regular C-prenylations and O-prenylations of aromatic substrates (2007), FEBS Lett., 581, 2889-2893.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)pLysS cells | Streptomyces virginiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | addition of EDTA (10 mM) to an assay not containing any divalent cations does not affect the enzyme activity | Streptomyces virginiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0021 | - |
geranyl diphosphate | with flaviolin as cosubstrate, in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae |  |
| 0.0027 | - |
geranyl diphosphate | with 1,3-dihydroxynaphthalene as cosubstrate, in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
| 0.021 | - |
flaviolin | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
| 1.9 | - |
1,3-dihydroxynaphthalene | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
| 5.6 | - |
4-hydroxybenzoate | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme activity is independent of the presence of Mg2+ or other divalent metal ions | Streptomyces virginiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32700 | - |
1 * 32700, calculated from amino acid sequence | Streptomyces virginiae |
| 33200 | - |
1 * 33200, SDS-PAGE | Streptomyces virginiae |
| 39100 | - |
gel filtration | Streptomyces virginiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate + flaviolin | Streptomyces virginiae | flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone | 3-linalylflaviolin + diphosphate | - |
? | |
| geranyl diphosphate + flaviolin | Streptomyces virginiae DSM 1042 | flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone | 3-linalylflaviolin + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces virginiae | - |
- |
- |
| Streptomyces virginiae DSM 1042 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA-agarose resin column chromatography, benzamidine-Sepharose column chromatography, and Superdex 200 gel filtration | Streptomyces virginiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranyl diphosphate + 1,3-dihydroxynaphthalene | - |
Streptomyces virginiae | 3-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-1-ol + 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-2-ol | - |
? | |
| geranyl diphosphate + 1,3-dihydroxynaphthalene | - |
Streptomyces virginiae DSM 1042 | 3-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-1-ol + 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]naphthalen-2-ol | - |
? | |
| geranyl diphosphate + 4-hydroxybenzoate | - |
Streptomyces virginiae | 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid | - |
? | |
| geranyl diphosphate + 4-hydroxybenzoate | - |
Streptomyces virginiae DSM 1042 | 4-[[(2E)-3,7-dimethylocta-2,6-dien-1-yl]oxy]benzoic acid | - |
? | |
| geranyl diphosphate + flaviolin | flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone | Streptomyces virginiae | 3-linalylflaviolin + diphosphate | - |
? | |
| geranyl diphosphate + flaviolin | flaviolin is 2,5,7-trihydroxy-1,4-naphthoquinone | Streptomyces virginiae DSM 1042 | 3-linalylflaviolin + diphosphate | - |
? | |
| additional information | does not accept 1,6-dihydroxynaphthalene and 4-hydroxyphenylpyruvate as substrate | Streptomyces virginiae | ? | - |
? | |
| additional information | does not accept 1,6-dihydroxynaphthalene and 4-hydroxyphenylpyruvate as substrate | Streptomyces virginiae DSM 1042 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33200, SDS-PAGE | Streptomyces virginiae |
| monomer | 1 * 32700, calculated from amino acid sequence | Streptomyces virginiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fnq26 | - |
Streptomyces virginiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
flaviolin | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
| 0.002 | - |
4-hydroxybenzoate | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
| 0.003 | - |
1,3-dihydroxynaphthalene | in 100 mM Na-TAPS (pH 8.5), at 30°C | Streptomyces virginiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Streptomyces virginiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.2 | half-maximal values at pH 7.5 and 9.2 | Streptomyces virginiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in FNQ I biosynthesis | Streptomyces virginiae |
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Release 2023.1 (January 2023)
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