KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
L-tyrosine | pH 8.0, 30°C | Leptosphaeria maculans | |
0.37 | - |
L-tryptophan | pH 8.0, 30°C | Leptosphaeria maculans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tyrosine | Leptosphaeria maculans | first committed step in the biosynthesis of the phytotoxin sirodesmin PL | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptosphaeria maculans | Q6Q874 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leptosphaeria maculans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | reverse prenylation | Leptosphaeria maculans | diphosphate + 1-(2-methylbut-3-en-2-yl)-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | catalytic efficiency for L-tryptophan is 6% compared to catalytic efficiency for L-tyrosine | Leptosphaeria maculans | diphosphate + 7-dimethylallyltryptophan | - |
? | |
dimethylallyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
dimethylallyl diphosphate + L-tyrosine | first committed step in the biosynthesis of the phytotoxin sirodesmin PL | Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
additional information | the catalytic erfficiency with 4-mercapto-L-phenylalanine, 4-methoxy-L-tryptophan and 7-methyl-L-tryptophan is less than 1% of the reaction with L-tyrosine | Leptosphaeria maculans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SirD | - |
Leptosphaeria maculans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Leptosphaeria maculans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
L-tryptophan | pH 8.0, 30°C | Leptosphaeria maculans | |
1.3 | - |
L-tyrosine | pH 8.0, 30°C | Leptosphaeria maculans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Leptosphaeria maculans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
L-tryptophan | pH 8.0, 30°C | Leptosphaeria maculans | |
4.3 | - |
L-tyrosine | pH 8.0, 30°C | Leptosphaeria maculans |