Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
DELTA319-323 | the deletion mutant shows increased catalytic efficiency for O-phospho-L-serine and reduced catalytic efficiency for [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | Mycobacterium tuberculosis | - |
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate | - |
? | |
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | Mycobacterium tuberculosis H37Rv | - |
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WP53 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WP53 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography and Sephadex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | - |
Mycobacterium tuberculosis | [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate | - |
? | |
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | - |
Mycobacterium tuberculosis H37Rv | [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
CysM | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.857 | - |
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | deletion mutant DELTA319-323, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.34 | - |
O-phospho-L-serine | wild type enzyme, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
8.85 | - |
O-phospho-L-serine | deletion mutant DELTA319-323, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
146 | - |
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | wild type enzyme, at pH 7.5 and 37°C | Mycobacterium tuberculosis |