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Literature summary for 2.5.1.113 extracted from

  • Agren, D.; Schnell, R.; Schneider, G.
    The C-terminal of CysM from Mycobacterium tuberculosis protects the aminoacrylate intermediate and is involved in sulfur donor selectivity (2009), FEBS Lett., 583, 330-336.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
DELTA319-323 the deletion mutant shows increased catalytic efficiency for O-phospho-L-serine and reduced catalytic efficiency for [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis
-
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis H37Rv
-
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WP53
-
-
Mycobacterium tuberculosis H37Rv P9WP53
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography and Sephadex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
Mycobacterium tuberculosis [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
Mycobacterium tuberculosis H37Rv [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
CysM
-
Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.857
-
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH deletion mutant DELTA319-323, at pH 7.5 and 37°C Mycobacterium tuberculosis
1.34
-
O-phospho-L-serine wild type enzyme, at pH 7.5 and 37°C Mycobacterium tuberculosis
8.85
-
O-phospho-L-serine deletion mutant DELTA319-323, at pH 7.5 and 37°C Mycobacterium tuberculosis
146
-
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH wild type enzyme, at pH 7.5 and 37°C Mycobacterium tuberculosis