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Literature summary for 2.5.1.112 extracted from
- Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases (2001), Plant Cell Physiol., 42, 677-685.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli AD494(DE3)pLysS cells | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0065 | - |
dimethylallyl diphosphate | in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C | Arabidopsis thaliana |  |
| 0.018 | - |
ATP | in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + ADP | Arabidopsis thaliana | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
| dimethylallyl diphosphate + ATP | Arabidopsis thaliana | highest activity with ATP | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + ADP | - |
Arabidopsis thaliana | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
| dimethylallyl diphosphate + ATP | highest activity with ATP | Arabidopsis thaliana | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
| additional information | no activity with AMP and tRNA | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dimethylallyl diphosphate:ATP/ADP isopentenyltransferase | - |
Arabidopsis thaliana |
| IPT4 | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Arabidopsis thaliana | |
| ATP | - |
Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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