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Literature summary for 2.5.1.112 extracted from

  • Sakano, Y.; Okada, Y.; Matsunaga, A.; Suwama, T.; Kaneko, T.; Ito, K.; Noguchi, H.; Abe, I.
    Molecular cloning, expression, and characterization of adenylate isopentenyltransferase from hop (Humulus lupulus L.) (2004), Phytochemistry, 65, 2439-2446.
    View publication on PubMed
Search for more literature for 2.5.1.112

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15[pREP4] cells Humulus lupulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0162
-
 ATP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
0.0193
-
 ADP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
0.0195
-
 dimethylallyl diphosphate in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
0.759
-
 AMP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36693
-
 x * 36693, calculated from amino acid sequence Humulus lupulus
37000
-
 x * 37000, SDS-PAGE Humulus lupulus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + ADP Humulus lupulus
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
dimethylallyl diphosphate + ATP Humulus lupulus ATP is the best cosubstrate diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Humulus lupulus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelate affinity column chromatography Humulus lupulus

Source Tissue

Source Tissue Comment Organism Textmining
cone
-
 Humulus lupulus
-
leaf
-
 Humulus lupulus
-
root
-
 Humulus lupulus
-
stem
-
 Humulus lupulus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + ADP
-
 Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
dimethylallyl diphosphate + AMP low activity with AMP Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine phosphate
-
 ?
dimethylallyl diphosphate + ATP ATP is the best cosubstrate Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?
additional information GMP, IMP, CMP, and UMP are not accepted as a substrate Humulus lupulus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Humulus lupulus
? x * 36693, calculated from amino acid sequence Humulus lupulus

Synonyms

Synonyms Comment Organism
adenylate isopentenyltransferase
-
 Humulus lupulus
AIPT
-
 Humulus lupulus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
 AMP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
0.022
-
 ATP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
0.024
-
 ADP in 100 mM Tris–HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
0.027
-
 dimethylallyl diphosphate in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus

Cofactor

Cofactor Comment Organism Structure
ADP preferred ligand over AMP Humulus lupulus
ATP preferred ligand over AMP Humulus lupulus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0109
-
 AMP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
1.238
-
 ADP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
1.34
-
 ATP in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus
1.397
-
 dimethylallyl diphosphate in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C Humulus lupulus