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Literature summary for 2.5.1.111 extracted from
- CloQ, a prenyltransferase involved in clorobiocin biosynthesis (2003), Proc. Natl. Acad. Sci. USA, 100, 2316-2321.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Streptomyces roseochromogenus subsp. oscitans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
4-hydroxyphenylpyruvate | pH 7.5, 30°C | Streptomyces roseochromogenus subsp. oscitans |  |
| 0.035 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Streptomyces roseochromogenus subsp. oscitans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the addition of Ca2+ (2.5 mM) instead of Mg2+ results in 70% of the activity obtained with Mg2+ | Streptomyces roseochromogenus subsp. oscitans | |
| Mg2+ | activates, 2.5 mM is the most effecive concentration. In the absence of divalent cations and in the presence of 5 mM EDTA, the enzyme retains 25% of its original activity. The enzyme is not dependent on the presence of Mg2+ | Streptomyces roseochromogenus subsp. oscitans | |
| additional information | both the zinc and the magnesium contents are below 0.1 mol per mol of CloQ | Streptomyces roseochromogenus subsp. oscitans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35600 | - |
1 * 35600, SDS-PAGE | Streptomyces roseochromogenus subsp. oscitans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | Streptomyces roseochromogenus subsp. oscitans | the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
| dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | Streptomyces roseochromogenus subsp. oscitans DS 12.976 | the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces roseochromogenus subsp. oscitans | Q8GHB2 | - |
- |
| Streptomyces roseochromogenus subsp. oscitans DS 12.976 | Q8GHB2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces roseochromogenus subsp. oscitans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin | Streptomyces roseochromogenus subsp. oscitans | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
| dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | the enzyme is specific for 4-hydroxyphenylpyruvate. CloQ does not accept prephenic acid, 4-hydroxybenzoate or 4-hydroxybenzaldehyde or as substrate. When dimethylallyl diphosphate is replaced with isopentenyl diphosphate or geranyl diphosphate, no product formation is observed | Streptomyces roseochromogenus subsp. oscitans | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
| dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | the enzyme is involved in the biosynthesis of the 3-dimethylallyl-4-hydroxyphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin | Streptomyces roseochromogenus subsp. oscitans DS 12.976 | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
| dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | the enzyme is specific for 4-hydroxyphenylpyruvate. CloQ does not accept prephenic acid, 4-hydroxybenzoate or 4-hydroxybenzaldehyde or as substrate. When dimethylallyl diphosphate is replaced with isopentenyl diphosphate or geranyl diphosphate, no product formation is observed | Streptomyces roseochromogenus subsp. oscitans DS 12.976 | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35600, SDS-PAGE | Streptomyces roseochromogenus subsp. oscitans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4HPP dimethylallyltransferase | - |
Streptomyces roseochromogenus subsp. oscitans |
| CloQ | - |
Streptomyces roseochromogenus subsp. oscitans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces roseochromogenus subsp. oscitans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Streptomyces roseochromogenus subsp. oscitans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the biosynthesis of the 4-hydroxy-3-dimethylallylphenylpyruvate moiety of the aminocoumarin antibiotic clorobiocin | Streptomyces roseochromogenus subsp. oscitans |
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