Application | Comment | Organism |
---|---|---|
synthesis | NovQ is capable of catalyzing the transfer of a dimethylallyl group to both phenylpropanoids, such as p-coumaric acid and caffeic acid, and the B-ring of flavonoids. NovQ can serve as a useful biocatalyst for the synthesis of prenylated phenylpropanoids and prenylated flavonoids | Streptomyces niveus |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli as an N-terminal His8-tagged protein | Streptomyces niveus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | even in the presence of 5mM EDTA, NovQ possessed full enzymatic activity | Streptomyces niveus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0151 | - |
4-hydroxyphenylpyruvate | pH 7.5, 30°C | Streptomyces niveus | |
0.0333 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Streptomyces niveus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | magnesium-independent prenyltransferase | Streptomyces niveus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Streptomyces niveus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | Streptomyces niveus | - |
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | Streptomyces niveus 16259 | - |
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces niveus | Q9L9F1 | - |
- |
Streptomyces niveus 16259 | Q9L9F1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces niveus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 1,6-dihydroxynaphthalene | 49.8% yield | Streptomyces niveus | diphosphate + 1-O-dimethylallyl-1,6-dihydroxynaphthalene | - |
? | |
dimethylallyl diphosphate + 1,6-dihydroxynaphthalene | 49.8% yield | Streptomyces niveus 16259 | diphosphate + 1-O-dimethylallyl-1,6-dihydroxynaphthalene | - |
? | |
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | 31% yield | Streptomyces niveus | diphosphate + 1-dimethylallyl-2,7-dihydroxynaphthalene | - |
? | |
dimethylallyl diphosphate + 2,7-dihydroxynaphthalene | 31% yield | Streptomyces niveus 16259 | diphosphate + 1-dimethylallyl-2,7-dihydroxynaphthalene | - |
? | |
dimethylallyl diphosphate + 4-coumarate | 16.8% yield | Streptomyces niveus | diphosphate + 4-O-dimethylallylcoumarate | - |
? | |
dimethylallyl diphosphate + 4-coumarate | 34.4% yield | Streptomyces niveus | diphosphate + 4-O-dimethylallylcoumarate | - |
? | |
dimethylallyl diphosphate + 4-coumarate | 16.8% yield | Streptomyces niveus 16259 | diphosphate + 4-O-dimethylallylcoumarate | - |
? | |
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | - |
Streptomyces niveus | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | - |
Streptomyces niveus 16259 | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
dimethylallyl diphosphate + apigenin | 7.6% yield | Streptomyces niveus | diphosphate + 3'-C-dimethylallyl apigenin | - |
? | |
dimethylallyl diphosphate + caffeate | 20% yield | Streptomyces niveus | diphosphate + 3-O,4-O-bis-(dimethylallyl)caffeate | - |
? | |
dimethylallyl diphosphate + caffeate | 17.3% yield | Streptomyces niveus | diphosphate + 3-O-(dimethylallyl)caffeate | - |
? | |
dimethylallyl diphosphate + caffeate | 26.1% yield | Streptomyces niveus | diphosphate + 4-O-(dimethylallyl)caffeate | - |
? | |
dimethylallyl diphosphate + naringenin | 87.2% yield | Streptomyces niveus | diphosphate + 3'-C-dimethylallyl naringenin | - |
? | |
dimethylallyl diphosphate + naringenin | 11.1% yield | Streptomyces niveus | diphosphate + 4'-O-dimethylallyl naringenin | - |
? | |
dimethylallyl diphosphate + resveratrol | 12.3% yield | Streptomyces niveus | diphosphate + 3'-dimethylallyl resveratrol | - |
? | |
additional information | no activity with olivetol, less than 5% yield with genistein, daidzein and 1,3-dihydroxynaphthalene | Streptomyces niveus | ? | - |
? | |
additional information | no activity with olivetol, less than 5% yield with genistein, daidzein and 1,3-dihydroxynaphthalene | Streptomyces niveus 16259 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Streptomyces niveus |
Synonyms | Comment | Organism |
---|---|---|
NovQ | - |
Streptomyces niveus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces niveus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
dimethylallyl diphosphate | pH 7.5, 30°C | Streptomyces niveus | |
0.083 | - |
4-hydroxyphenylpyruvate | pH 7.5, 30°C | Streptomyces niveus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces niveus |