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Literature summary for 2.5.1.101 extracted from

  • Schoenhofen, I.; Vinogradov, E.; Whitfield, D.; Brisson, J.; Logan, S.
    The CMP-legionaminic acid pathway in Campylobacter: Biosynthesis involving novel GDP-linked precursors (2009), Glycobiology, 19, 715-725.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene legI or Cj1327, DNA and ammino acid sequence determination and analysis, expression as N-terminally His6-tagged enzyme Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O Campylobacter jejuni
-
N,N'-diacetyllegionaminate + phosphate
-
?
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O Campylobacter jejuni 11168
-
N,N'-diacetyllegionaminate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
gene legI or Cj1327
-
Campylobacter jejuni 11168
-
gene legI or Cj1327
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
Campylobacter jejuni N,N'-diacetyllegionaminate + phosphate
-
?
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
Campylobacter jejuni 11168 N,N'-diacetyllegionaminate + phosphate
-
?

Synonyms

Synonyms Comment Organism
legI
-
Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Campylobacter jejuni

General Information

General Information Comment Organism
metabolism the enzyme is part of the biosynthesis of legionaminic acid and its CMP-activated form, metabolite determination and identification by NMR spectroscoopy for pathway construction and analysis, overview Campylobacter jejuni