Cloned (Comment) | Organism |
---|---|
gene legI or Cj1327, DNA and ammino acid sequence determination and analysis, expression as N-terminally His6-tagged enzyme | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O | Campylobacter jejuni | - |
N,N'-diacetyllegionaminate + phosphate | - |
? | |
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O | Campylobacter jejuni 11168 | - |
N,N'-diacetyllegionaminate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
gene legI or Cj1327 | - |
Campylobacter jejuni 11168 | - |
gene legI or Cj1327 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O | - |
Campylobacter jejuni | N,N'-diacetyllegionaminate + phosphate | - |
? | |
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O | - |
Campylobacter jejuni 11168 | N,N'-diacetyllegionaminate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
legI | - |
Campylobacter jejuni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Campylobacter jejuni |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of the biosynthesis of legionaminic acid and its CMP-activated form, metabolite determination and identification by NMR spectroscoopy for pathway construction and analysis, overview | Campylobacter jejuni |