Cloned (Comment) | Organism |
---|---|
expresion in Escherichia coli after truncation of the signal sequence at the 5'-end of the coding region | Phaedon cochleariae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isopentenyl diphosphate | substrate inhibition | Phaedon cochleariae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00084 | - |
isopentenyl diphosphate | fixed substrate dimethylallyl diphosphate (0.05 mM), pH and temperature not specified in the publication, metal cofactor: Co2+ | Phaedon cochleariae | |
0.0116 | - |
dimethylallyl diphosphate | fixed substrate isopentenyl diphosphate (0.015 mM), pH and temperature not specified in the publication, metal cofactor: Co2+ | Phaedon cochleariae | |
0.0118 | - |
isopentenyl diphosphate | fixed substrate dimethylallyl diphosphate (0.05 mM), pH and temperature not specified in the publication, metal cofactor: Mg2+ | Phaedon cochleariae | |
1.103 | - |
dimethylallyl diphosphate | fixed substrate isopentenyl diphosphate (0.015 mM), pH and temperature not specified in the publication, metal cofactor: Mg2+ | Phaedon cochleariae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | with dimethylallyl diphosphate and isopentenyl diphosphate the enzyme is far more active with Co2+ as an additive than with any other tested metal ion. In the presence of Co2+ or Mn2+, with dimethylallyl diphosphate as a cosubstrate, the enzyme produces about 96% geranyl diphosphate and only 4% farnesyl diphosphate. In contrast, with Mg2+ as an additive, PcIDS1 produces 18% geranyl diphosphate and 82% farnesyl diphosphate | Phaedon cochleariae | |
Mg2+ | with dimethylallyl diphosphate and isopentenyl diphosphate the enzyme is far more active with Co2+ as an additive than with any other tested metal ion. In the presence of Co2+ or Mn2+, with dimethylallyl diphosphate as a cosubstrate, the enzyme produces about 96% geranyl diphosphate and only 4% farnesyl diphosphate. In contrast, with Mg2+ as an additive, PcIDS1 produces 18% geranyl diphosphate and 82% farnesyl diphosphate | Phaedon cochleariae | |
additional information | the enzyme is inactive without adding divalent metal cations | Phaedon cochleariae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45800 | - |
2 * 45800, the enzyme is always present as a dimer regardless of the added cofactor, in the case of Mg2+, the dimeric protein possesses the largest volume (93800 Da), most likely due to a more relaxed conformation. With Co2+, the enzyme seems to have a more compact conformation (87600 Da), which may be responsible for the change in product spectrum calculated from sequence | Phaedon cochleariae |
87600 | - |
enzyme-Co2+ complex, gel filtration | Phaedon cochleariae |
93800 | - |
enzyme-Mg2+ complex, gel filtration | Phaedon cochleariae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + isopentenyl diphosphate | Phaedon cochleariae | the enzyme is involved in the biosynthesis of the monoterpenoid precursors needed for formation of the defensive compound chrysomelidial. Geranyl diphosphate is needed for the de novo synthesis of the cyclopentanoid monoterpene iridoids, defensive compounds that are produced during the entire larval stage of Phaedon cochleariae | diphosphate + geranyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaedon cochleariae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phaedon cochleariae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + isopentenyl diphosphate | the enzyme is involved in the biosynthesis of the monoterpenoid precursors needed for formation of the defensive compound chrysomelidial. Geranyl diphosphate is needed for the de novo synthesis of the cyclopentanoid monoterpene iridoids, defensive compounds that are produced during the entire larval stage of Phaedon cochleariae | Phaedon cochleariae | diphosphate + geranyl diphosphate | - |
? | |
dimethylallyl diphosphate + isopentenyl diphosphate | the enzyme is bifunctional and produces (2E,6E)-farnesyl diphosphate or geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate depending on the divalent metal cofactor present. The enzyme is far more active with Co2+ as an additive than with any other tested metal ion. In the presence of Co2+ or Mn2+, with dimethylallyl diphosphate as a cosubstrate, it produces about 96% geranyl diphosphate and only 4% farnesyl diphosphate. In contrast, with Mg2+ as an additive, the enzyme produces 18% geranyl diphosphate and farnesyl diphosphate | Phaedon cochleariae | diphosphate + geranyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45800, the enzyme is always present as a dimer regardless of the added cofactor, in the case of Mg2+, the dimeric protein possesses the largest volume (93800 Da), most likely due to a more relaxed conformation. With Co2+, the enzyme seems to have a more compact conformation (87600 Da), which may be responsible for the change in product spectrum calculated from sequence | Phaedon cochleariae |
Synonyms | Comment | Organism |
---|---|---|
PcIDS1 | - |
Phaedon cochleariae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.573 | - |
isopentenyl diphosphate | fixed substrate dimethylallyl diphosphate (0.05 mM), pH and temperature not specified in the publication, metal cofactor: Mg2+ | Phaedon cochleariae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Phaedon cochleariae | calculated from sequence | - |
8.63 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the monoterpenoid precursors needed for formation of the defensive compound chrysomelidial | Phaedon cochleariae |