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Literature summary for 2.4.99.B5 extracted from

  • Leemhuis, H.; Wehmeier, U.F.; Dijkhuizen, L.
    Single amino acid mutations interchange the reaction specificities of cyclodextrin glycosyltransferase and the acarbose-modifying enzyme acarviosyl transferase (2004), Biochemistry, 43, 13204-13213.
    View publication on PubMed
Search for more literature for 2.4.99.B5

Protein Variants

Protein Variants Comment Organism
D229N mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity Actinoplanes sp.
D328N mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity Actinoplanes sp.
E257Q mutant enzyme without acarviosyl transferase activity and 4-alpha-glucanotransferase activity Actinoplanes sp.
G140H mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 2% of starch into cyclodextrins Actinoplanes sp.
G140H mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is strongly enhanced Actinoplanes sp.
G140H/Q327H mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 1.2% of starch into cyclodextrins Actinoplanes sp.
G140H/Q327H/T329M mutation changes acarviosyl transferase into an enzyme with 4-alpha-glucanotransferase activity. This mutation introduces cyclodextrin-forming activity into acarviosyl transferase, converting 7.4% of starch into cyclodextrins Actinoplanes sp.
Q327H mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is reduced Actinoplanes sp.
T329M mutation decreases acarviosyl transferase activity, 4-alpha-glucanotransferase activity is strongly enhanced Actinoplanes sp.

Organism

Organism UniProt Comment Textmining
Actinoplanes sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
 pH 6.5, 30°C Actinoplanes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acarbose + maltotriose
-
 Actinoplanes sp. acarviosyl-alpha-(1->4)-maltotriose + maltose
-
 ?
additional information the enzyme is inactive on starch Actinoplanes sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
ATASE
-
 Actinoplanes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Actinoplanes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Actinoplanes sp.