Literature summary for 2.4.99.18 extracted from

Wacker, M.; Feldman, M.F.; Callewaert, N.; Kowarik, M.; Clarke, B.R.; Pohl, N.L.; Hernandez, M.; Vines, E.D.; Valvano, M.A.; Whitfield, C.; Aebi, M.
Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems (2006), Proc. Natl. Acad. Sci. USA, 103, 7088-7093.

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Cloned(Commentary)

Cloned (Comment)	Organism
functionally expressed in Escherichia coli	Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dolichyl diphosphooligosaccharide + [protein]-L-asparagine	Campylobacter jejuni	-	dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine	-	?

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dolichyl diphosphooligosaccharide + [protein]-L-asparagine	-	Campylobacter jejuni	dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine	-	?
dolichyl diphosphooligosaccharide + [protein]-L-asparagine	to investigate the substrate specificity of PglB, the transfer of a set of lipid-linked polysaccharides in Escherichia coli and Salmonella enterica serovar typhimurium is tested. A hexose linked to the C-6 of the monosaccharide at the reducing end does not inhibit the transfer of the O antigen to the acceptor protein. PglB requires an acetamido group at the C-2. A model for the mechanism of PglB involving this functional group is proposed. Eukaryotic and prokaryotic OTases catalyze the transfer of oligosaccharides by a conserved mechanism. Substitution at the C-6 position in the reducing end of the oligosaccharide does not prevent its PglB-mediated transfer to the protein acceptor AcrA. PglB transferrs a polysaccharide that is assembled by a Wzy-protein-independent pathway. An N-acetyl group in position 2 of the sugar directly linked to the undecaprenyl diphosphate carrier is necessary for recognition and/or catalysis	Campylobacter jejuni	dolichyl diphosphate + [protein]-N-oligosaccharidyl-L-asparagine	-	?

Synonyms

Synonyms	Comment	Organism
OTase	-	Campylobacter jejuni
PglB	-	Campylobacter jejuni
PglB oligosaccharyltransferase	-	Campylobacter jejuni

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Release 2023.1 (January 2023)