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Literature summary for 2.4.99.18 extracted from

  • Kumar, V.; Heinemann, F.S.; Ozols, J.
    Interleukin-2 induces N-glycosylation in T-cells: characterization of human lymphocyte oligosaccharyltransferase (1998), Biochem. Biophys. Res. Commun., 247, 524-529.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
Digitonin purified enzyme requires 0.1% digitonin for maximal activity Homo sapiens
phosphatidylcholine optimal concentration: 2 mM Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Digitonin above 0.1% Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
lymphocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dolichyl diphosphooligosaccharide + protein L-asparagine
-
 Homo sapiens dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine
-
 ?

Subunits

Subunits Comment Organism
More after SDS-PAGE the most active fractions contain four predominant protein bands with apparent molecular weight in the 50000 Da to 65000 Da range. N-terminal sequence analysis identifies the protein as ribophorin I, ribophorin II, and a 50000 Da homologue of Wbp1, a yeast protein essential for N-glycosylation Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 3 h, stable Homo sapiens