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Literature summary for 2.4.99.18 extracted from
- N-Glycosylation of yeast proteins. Characterization of the solubilized oligosaccharyl transferase (1981), Eur. J. Biochem., 116, 101-108.
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 25%, stabilizes | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| octylglucoside | concentration 1% | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
dolichyl diphosphochitobiose-(Man)9-(Glc)3 | - |
Saccharomyces cerevisiae | |
| 0.0012 | - |
dolichyl diphosphochitobiose | - |
Saccharomyces cerevisiae | |
| 0.05 | - |
Tyr-Asn-Leu-Thr-Ser-Val | reaction with dolichyl diphosphochitobiose-Man9Glc3 | Saccharomyces cerevisiae | |
| 0.6 | - |
Tyr-Asn-Leu-Thr-Ser-Val | reaction with dolichyl diphosphochitobiose | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound to | Saccharomyces cerevisiae | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent cation required. Mg2+, at concentration of 10 mM yields only one third the activity observed with Mn2+ | Saccharomyces cerevisiae | |
| Mn2+ | divalent cation required, 1 mM Mn2+ is optimal | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
wild-type strain X-2180-1A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, Nonidet-solubilized crude enzyme, 25% glycerol, 0.01% 2-mercaptoethanol, 18% loss of activity after 1 month and 87% after 5 months | Saccharomyces cerevisiae |
| -70°C, Nonidet-solubilized crude enzyme, 25% glycerol, 0.01% 2-mercaptoethanol, retains 90% of original activity after 1 month and 55% after 5 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl diphosphate-di-N-acetylchitobiose + Tyr-Asn-Leu-Thr-Ser-Val | - |
Saccharomyces cerevisiae | ? | - |
? | |
| dolichyl diphosphochitobiose-(mannosyl)9-(glucosyl)3 + synthetic hexapeptide | glycosyl-donors are also dolichyl diphosphochitobiose or dolichyl diphospho-chitobiose-mannose, no substrates are dolichyl diphosphochitobiose-(mannosyl)9 or dolichyl diphospho-N-acetyl-D-glucosamine | Saccharomyces cerevisiae | ? | - |
? | |
| dolichyl diphosphochitobiose-(mannosyl)9-(glucosyl)3 + synthetic hexapeptide | e.g. Tyr-Asn-Leu-Thr-Ser-Val | Saccharomyces cerevisiae | ? | - |
? | |
| dolichyl diphosphooligosaccharide + protein L-asparagine | glycosyl donor specificity | Saccharomyces cerevisiae | dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine | - |
? | |
| dolichyl-diphosphochitobiose + Tyr-Asn-Leu-Thr-Ser-Val | - |
Saccharomyces cerevisiae | ? | - |
? | |
| dolichyl-diphosphochitobiose-Man1 + Tyr-Asn-Leu-Thr-Ser-Val | - |
Saccharomyces cerevisiae | ? | - |
? | |
| dolichyl-diphosphochitobiose-Man9Glc3 + Tyr-Asn-Leu-Thr-Ser-Val | - |
Saccharomyces cerevisiae | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.7 | - |
Saccharomyces cerevisiae |
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