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Literature summary for 2.4.99.17 extracted from

  • Slany, R.K.; Boesl, M.; Crain, P.F.; Kersten, H.
    A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine (1993), Biochemistry, 32, 7811-7817.
    View publication on PubMed
Search for more literature for 2.4.99.17

Cloned(Commentary)

Cloned (Comment) Organism
the enzyme is overexpressed as a fusion protein with the glutathione S-transferase from Schistosoma japonicum in Escherichia coli strain DH5alpha Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 1 * 39000, native enzyme, estimated from amino acid sequence Escherichia coli
40000
-
 native enzyme, gel filtration Escherichia coli
65000
-
 1 * 65000, His-tagged enzyme, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, glutathione-Sepharose column chromatography, and MonoQ anion-exchange column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine34 in tRNA
-
 Escherichia coli L-methionine + adenine + epoxyqueuosine34 in tRNA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 39000, native enzyme, estimated from amino acid sequence Escherichia coli
monomer 1 * 65000, His-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
QueA
-
 Escherichia coli
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
-
 Escherichia coli