Crystallization (Comment) | Organism |
---|---|
alpha-maltose 1-phosphate bound to a D394A mutant and beta-2-deoxy-2-fluoromaltosyl-enzyme intermediate with a E423A mutant is crystallized with 15% (w/v) polyethylene glycol 3350, 0.2 M sodium citrate, and 15% (w/v) ethylene glycol | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
D394A | the maltosyl transferase activity of the mutant is more than 4 orders of magnitude lower than that of the wild type protein | Streptomyces coelicolor |
E423A | the mutant turns over alpha-maltose 1-phosphate about 500times slower than the wild type enzyme | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-2-fluoro-alpha-maltosyl fluoride | after preincubation for 5 min with 1 mM substrate analogue 2-deoxy-2-fluoro-alpha-maltosyl fluoride, 70% of the normal activity with alpha-maltose 1-phosphate is lost | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n | Streptomyces coelicolor | - |
phosphate + [(1->4)-alpha-D-glucosyl]n+2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9L1K2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-2-fluoro-alpha-maltosyl fluoride + maltotetraose | - |
Streptomyces coelicolor | ? | - |
? | |
alpha-maltose 1-phosphate + [(1->4)-alpha-D-glucosyl]n | - |
Streptomyces coelicolor | phosphate + [(1->4)-alpha-D-glucosyl]n+2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
alpha-maltose 1-phosphate:(1->4)-alpha-D-glucan 4-alpha-D-maltosyltransferase | - |
Streptomyces coelicolor |
GlgE isoform I | - |
Streptomyces coelicolor |
maltosyl transferase | - |
Streptomyces coelicolor |