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Literature summary for 2.4.99.12 extracted from

  • Schmidt, H.; Hansen, G.; Singh, S.; Hanuszkiewicz, A.; Lindner, B.; Fukase, K.; Woodard, R.; Holst, O.; Hilgenfeld, R.; Mamat, U.; Mesters, J.
    Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis (2012), Proc. Natl. Acad. Sci. USA, 109, 6253-6258.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.99.12

Crystallization (Commentary)

Crystallization (Comment) Organism
substrate-free form and in complex with CMP, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.5), 35-40% (v/v) PEG 400, 200 mM Na-citrate, 50 mM 2-mercaptoethanol, at 19°C Aquifex aeolicus

Protein Variants

Protein Variants Comment Organism
E276A the mutation causes a decrease in the catalytic activity of the enzyme Aquifex aeolicus
G30A the mutant is completely inactive Aquifex aeolicus
H272A/N273A the mutations drastically affect the ability of the enzyme to transfer Kdo Aquifex aeolicus
R212A the mutation clearly affects but does not completely prevent Kdo transfer activity Aquifex aeolicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Aquifex aeolicus 16020
-

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O66663
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Heparin-Sepharose column chromatography Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tetraacyl-1,4-bisphosphate lipid A precursor 406 + CMP-alpha-Kdo
-
 Aquifex aeolicus CMP + ?
-
 ?

Synonyms

Synonyms Comment Organism
Kdo transferase
-
 Aquifex aeolicus
WaaAAAE
-
 Aquifex aeolicus