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Literature summary for 2.4.2.9 extracted from

  • Dai, Y.P.; Lee, C.S.; O'Sullivan, W.J.
    Properties of uracil phosphoribosyltransferase from Giardia intestinalis (1995), Int. J. Parasitol., 25, 207-214.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dGTP highly activating, no effect on Km values Giardia intestinalis
GTP highly activating, no effect on Km values Giardia intestinalis

General Stability

General Stability Organism
1 mM DTT and 0.1 mM EDTA stabilize the purified enzyme Giardia intestinalis

Inhibitors

Inhibitors Comment Organism Structure
1,3-dimethyl-uracil
-
Giardia intestinalis
5-acetoxyuracil
-
Giardia intestinalis
5-Bromouracil
-
Giardia intestinalis
5-fluoro-deoxyuracil
-
Giardia intestinalis
5-n-propyl-2'-deoxyuridine
-
Giardia intestinalis
5-Nitrouracil
-
Giardia intestinalis
6-benzyl-2-thiouracil
-
Giardia intestinalis
6-formyluracil
-
Giardia intestinalis
diphosphate noncompetitive against uracil, competitive against 5-phosphoribose 1-diphosphate Giardia intestinalis
DTNB 4 mM, 87% inhibition after 30 min Giardia intestinalis
tetrahydrouridine
-
Giardia intestinalis
UMP competitive against uracil, noncompetitive against Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
Uracil
-
Giardia intestinalis
0.2
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Giardia intestinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Giardia intestinalis
Cd2+ activates Giardia intestinalis
Co2+
-
Giardia intestinalis
divalent cations preference of activation by divalent cations in decreasing order: Mn2+, Co2+, Cd2+, Mg2+, Ni2+, Ca2+, all 1 mM Giardia intestinalis
Mg2+
-
Giardia intestinalis
Mn2+ required, 10 mM Giardia intestinalis
Mn2+ better activator than Mg2+ Giardia intestinalis
additional information no inhibition by any metal ion up to 10 mM Giardia intestinalis
Ni2+ activates Giardia intestinalis
Zn2+
-
Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, SDS-PAGE Giardia intestinalis
76000
-
gel filtration Giardia intestinalis

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-
Giardia intestinalis Portland
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Giardia intestinalis

Reaction

Reaction Comment Organism Reaction ID
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate rapid-random equilibrium mechanism Giardia intestinalis

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
Giardia intestinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1
-
purified enzyme Giardia intestinalis

Storage Stability

Storage Stability Organism
-20°C, 20 mM Hepes-KOH, pH 7.4, 1 mM DTT, stable for 3 days Giardia intestinalis
-70°C, 20 mM Hepes-KOH, pH 7.4, 1-2 months without loss of activity Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
Giardia intestinalis UMP + diphosphate
-
?
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
Giardia intestinalis Portland UMP + diphosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Giardia intestinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Giardia intestinalis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5
-
Giardia intestinalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.015
-
UMP competitive versus uracil Giardia intestinalis
0.017
-
tetrahydrouridine
-
Giardia intestinalis
0.24
-
diphosphate competitive versus 5-phosphoribose 1-diphosphate Giardia intestinalis