Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dGTP | highly activating, no effect on Km values | Giardia intestinalis | |
GTP | highly activating, no effect on Km values | Giardia intestinalis |
General Stability | Organism |
---|---|
1 mM DTT and 0.1 mM EDTA stabilize the purified enzyme | Giardia intestinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-dimethyl-uracil | - |
Giardia intestinalis | |
5-acetoxyuracil | - |
Giardia intestinalis | |
5-Bromouracil | - |
Giardia intestinalis | |
5-fluoro-deoxyuracil | - |
Giardia intestinalis | |
5-n-propyl-2'-deoxyuridine | - |
Giardia intestinalis | |
5-Nitrouracil | - |
Giardia intestinalis | |
6-benzyl-2-thiouracil | - |
Giardia intestinalis | |
6-formyluracil | - |
Giardia intestinalis | |
diphosphate | noncompetitive against uracil, competitive against 5-phosphoribose 1-diphosphate | Giardia intestinalis | |
DTNB | 4 mM, 87% inhibition after 30 min | Giardia intestinalis | |
tetrahydrouridine | - |
Giardia intestinalis | |
UMP | competitive against uracil, noncompetitive against | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
Uracil | - |
Giardia intestinalis | |
0.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Giardia intestinalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Giardia intestinalis | |
Cd2+ | activates | Giardia intestinalis | |
Co2+ | - |
Giardia intestinalis | |
divalent cations | preference of activation by divalent cations in decreasing order: Mn2+, Co2+, Cd2+, Mg2+, Ni2+, Ca2+, all 1 mM | Giardia intestinalis | |
Mg2+ | - |
Giardia intestinalis | |
Mn2+ | required, 10 mM | Giardia intestinalis | |
Mn2+ | better activator than Mg2+ | Giardia intestinalis | |
additional information | no inhibition by any metal ion up to 10 mM | Giardia intestinalis | |
Ni2+ | activates | Giardia intestinalis | |
Zn2+ | - |
Giardia intestinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, SDS-PAGE | Giardia intestinalis |
76000 | - |
gel filtration | Giardia intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | - |
- |
- |
Giardia intestinalis Portland | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Giardia intestinalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate | rapid-random equilibrium mechanism | Giardia intestinalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Giardia intestinalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.1 | - |
purified enzyme | Giardia intestinalis |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Hepes-KOH, pH 7.4, 1 mM DTT, stable for 3 days | Giardia intestinalis |
-70°C, 20 mM Hepes-KOH, pH 7.4, 1-2 months without loss of activity | Giardia intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Giardia intestinalis | UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Giardia intestinalis Portland | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Giardia intestinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Giardia intestinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Giardia intestinalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Giardia intestinalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
UMP | competitive versus uracil | Giardia intestinalis | |
0.017 | - |
tetrahydrouridine | - |
Giardia intestinalis | |
0.24 | - |
diphosphate | competitive versus 5-phosphoribose 1-diphosphate | Giardia intestinalis |