Any feedback?
Literature summary for 2.4.2.9 extracted from
- Characterization of a partially purified uracil phosphoribosyltransferase from the opportunistic pathogen Candida albicans (1994), Mycopathologia, 125, 129-141.
General Stability
| General Stability | Organism |
|---|---|
| enzyme is extremly unstable, UMP stabilizes during storage | Candida albicans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0705 | - |
Uracil | - |
Candida albicans |  |
| 0.186 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Candida albicans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Candida albicans | |
| Mg2+ | optimal concentration : 2 mM | Candida albicans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 47000 + x * 38000, SDS-PAGE | Candida albicans |
| 47000 | - |
x * 47000 + x * 38000, SDS-PAGE | Candida albicans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| only partial, due to the labile nature of the enzyme further purification is unsuccessful | Candida albicans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.068 | - |
partially purified enzyme | Candida albicans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT, 1 mM UMP, loss of 40% activity in 1 week | Candida albicans |
| 4°C, Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT, loss of 70% activity in 4 days | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | 1.6fold higher specific activity compared to uracil | Candida albicans | 5-fluoro-UMP + diphosphate | - |
? | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Candida albicans | UMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000 + x * 38000, SDS-PAGE | Candida albicans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
4 min, complete loss of activity | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Candida albicans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
