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Literature summary for 2.4.2.9 extracted from
- Purification and some properties of uracil phosphoribosyltransferase from Escherichia coli K12 (1986), Biochim. Biophys. Acta, 881, 268-275.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GTP | effect is pH-dependent, pH 7.5: lowers Km value for 5-phospho-alpha-D-ribose 1-diphosphate and increases Vmax 2fold, no effect on Km for uracil, pH 8.0: no effect | Escherichia coli |  |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol, not essential for stability in long-term storage | Escherichia coli |
| ethylene glycol, significantly increases stability with virtually no loss of activity after storage at 4°C for 13 days or after 1 year at -70°C | Escherichia coli |
| GTP, 5 mM with 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 2 mM 2-mercaptoethanol, labilizes with only 15% activity remaining after 13 days, labilizing effect abolished in presence of 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by diphosphate | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
Uracil | - |
Escherichia coli | |
| 0.3 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23500 | - |
3 * 23500, SDS-PAGE | Escherichia coli |
| 75000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli | pyrimidine salvage enzyme, no de novo synthesis of pyrmidines | UMP + diphosphate | - |
? | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli SO 1344 | pyrimidine salvage enzyme, no de novo synthesis of pyrmidines | UMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 | - |
| Escherichia coli | - |
gene upp | - |
| Escherichia coli | - |
and strain SO 1346 | - |
| Escherichia coli | - |
enzyme expression is inducible upon uracil starvation | - |
| Escherichia coli SO 1344 | - |
and strain SO 1346 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from strain SO 1346 | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.192 | - |
strain SO 1346 | Escherichia coli |
| 6.602 | - |
purified enzyme, strain SO 1346 | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C for 13 days or 1 year at -70°C, ethylene glycol, significantly increases stability with virtually no loss of activity | Escherichia coli |
| 4°C, 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 2 mM 2-mercaptoethanol, 44% loss of activity after 8 d | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | 216% of the activity with uracil | Escherichia coli | 5-fluoro-UMP + diphosphate | - |
r | |
| 6-azauracil + 5-phospho-alpha-D-ribose 1-diphosphate | 97% of the activity with uracil | Escherichia coli | 6-aza-UMP + diphosphate | - |
? | |
| 6-azauracil + 5-phospho-alpha-D-ribose 1-diphosphate | 97% of the activity with uracil | Escherichia coli SO 1344 | 6-aza-UMP + diphosphate | - |
? | |
| additional information | no substrates: thymine, hypoxanthine | Escherichia coli | ? | - |
? | |
| additional information | no substrates: cytosine, orotic acid | Escherichia coli | ? | - |
? | |
| additional information | no substrates: thymine, hypoxanthine | Escherichia coli SO 1344 | ? | - |
? | |
| additional information | no substrates: cytosine, orotic acid | Escherichia coli SO 1344 | ? | - |
? | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | highly specific for uracil and analogues | Escherichia coli | UMP + diphosphate | - |
r | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | pyrimidine salvage enzyme, no de novo synthesis of pyrmidines | Escherichia coli | UMP + diphosphate | - |
? | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | highly specific for uracil and analogues | Escherichia coli SO 1344 | UMP + diphosphate | - |
r | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | pyrimidine salvage enzyme, no de novo synthesis of pyrmidines | Escherichia coli SO 1344 | UMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 23500, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Escherichia coli |
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