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Literature summary for 2.4.2.7 extracted from
- Adenine phosphoribosyltransferase isoforms of Arabidopsis and their potential contributions to adenine and cytokinin metabolism (2002), Physiol. Plant., 115, 56-68.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of the 3 isoforms in Escherichia coli Bl21(DE3)pLysS as His-tagged proteins, the majority of the recombiannt enzyme is in the soluble fraction | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
adenine | APT3, pH 7.4 | Arabidopsis thaliana |  |
| 0.001 | - |
adenine | APT1, pH 7.4 | Arabidopsis thaliana | |
| 0.0026 | - |
adenine | APT2, pH 7.4 | Arabidopsis thaliana | |
| 0.015 | - |
benzyladenine | - |
Arabidopsis thaliana | |
| 0.076 | - |
zeatin | - |
Arabidopsis thaliana | |
| 0.11 | - |
isopentenyladenine | - |
Arabidopsis thaliana | |
| 0.33 | - |
zeatin | - |
Arabidopsis thaliana | |
| 0.44 | - |
benzyladenine | - |
Arabidopsis thaliana | |
| 0.44 | - |
isopentenyladenine | - |
Arabidopsis thaliana | |
| 1.8 | - |
zeatin | - |
Arabidopsis thaliana | |
| 2.4 | - |
benzyladenine | - |
Arabidopsis thaliana | |
| 2.5 | - |
isopentenyladenine | - |
Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
| cytosol | - |
Arabidopsis thaliana | 5829 | - |
| nucleus | - |
Arabidopsis thaliana | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, SDS-PAGE | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | P31166 | isoform APT1 | - |
| Arabidopsis thaliana | Q42563 | isoform APT2 | - |
| Arabidopsis thaliana | Q9LFP0 | isoform APT5 | - |
| Arabidopsis thaliana | Q9SU38 | isoform APT4 | - |
| Arabidopsis thaliana | Q9SUW2 | isoform APT3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 isoforms recombinant from Escherichia coli as His-tagged proteins | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | best acceptor substrate | Arabidopsis thaliana | AMP + diphosphate | - |
? | |
| benzyladenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | benzyladenosine 5'-phosphate + diphosphate | - |
? | |
| isopentenyladenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | isopentenyladenosine 5'-phosphate + diphosphate | - |
? | |
| zeatin + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | zeatin riboside 5'-phosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.5 | APT2 and 3, 37°C | Arabidopsis thaliana |
| 8.8 | - |
APT1, 37°C | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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