General Stability | Organism |
---|---|
ammonium sulfate, 1 M, partially protects against inactivation during storage | Homo sapiens |
dithiothreitol, 10 mM, or dimethylsulfoxide, 5%, or 5-phospho-alpha-D-ribose 1-diphosphate, 1 mM, protects against inactivation during storage | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11100 | - |
3 * 11100, SDS-PAGE | Homo sapiens |
34000 | - |
gel filtration, sucrose density gradient ultracentrifugation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.58 | - |
purified enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, purified enzyme, 5 mM MgCl2, 0.1 mM 5-phospho-alpha-D-ribose 1-diphosphate, stable for upt to 2 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-diaminopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 2,6-diaminopurine ribotide + diphosphate | - |
? | |
4-amino-5-imidazolecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 4-amino-5-imidazolecarboxamide ribotide + diphosphate | - |
? | |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | best acceptor substrate | Homo sapiens | AMP + diphosphate | - |
? | |
additional information | no donor substrates: D-ribose 5-phosphate, ribose 1-phosphate | Homo sapiens | ? | - |
? | |
additional information | at 0°C in the absence of Mg2+ but in presence of substrates the enzyme catalyzes a rapid and limited synthesis of AMP | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 11100, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
at 0°C in the absence of Mg2+ but in presence of substrates enzyme catalyzes a rapid and limited synthesis of AMP | Homo sapiens |
37 | - |
assay at | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9.5 | broad maximum | Homo sapiens |