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Literature summary for 2.4.2.7 extracted from

  • Groth, D.P.; Young, L.G.; Kenimer, J.G.
    Adenine phosphoribosyltransferase from rat liver (1978), Methods Enzymol., 51, 574-580.
    View publication on PubMed
Search for more literature for 2.4.2.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Rattus norvegicus
0.0008 0.001 adenine
-
 Rattus norvegicus
0.005
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17500
-
 x * 17500, SDS-PAGE Rattus norvegicus
22000
-
 sucrose density gradient sedimentation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
 purified enzyme Rattus norvegicus

Storage Stability

Storage Stability Organism
2-4°C, purified enzyme in the ampholine gradient solution, half-life of 2-4 weeks, dilution inactivates Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Rattus norvegicus AMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 17500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 active over a broad range increasing progressively in activity from pH 5.5-10 Rattus norvegicus