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Literature summary for 2.4.2.7 extracted from
- Adenine phosphoribosyltransferase from Escherichia coli (1978), Methods Enzymol., 51, 558-567.
General Stability
| General Stability | Organism |
|---|---|
| instability after calcium phosphate gel treatment | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Escherichia coli |  |
| Ba2+ | - |
Escherichia coli | |
| diphosphate | - |
Escherichia coli | |
| nucleotides | higher concentrations of all 5'-nucleotides are most inhibitory, 6-OH purine nucleotides are moderately inhibitory, pyrimidine nucleotides are least inhibitory | Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Escherichia coli | |
| 0.02 | - |
adenine | - |
Escherichia coli | |
| 0.125 | - |
5-phospho-alpha-D-ribose 1-diphosphate | in presence of a 2fold excess of Mg2+ | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibition | Escherichia coli | |
| Mg2+ | required | Escherichia coli | |
| Mg2+ | absolute requirement | Escherichia coli | |
| Mg2+ | competitive to 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli | |
| Mg2+ | enzyme requires Mn2+ or Mg2+ | Escherichia coli | |
| Mg2+ | maximal activity if Mg2+ is twice the concentration of 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli | |
| additional information | activity absolutely depends on presence of divalent cations | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli | enzyme mediates the translocation of adenine into the cell as AMP | AMP + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli | necessary for appropriate regulation of purine de novo biosynthesis | AMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate | ping-pong mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14 | - |
purified enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme, 2-3 weeks, considerable loss of activity | Escherichia coli |
| -79°C, purified enzyme, 0.1 mg/ml protein, 0.1 M potassium phosphate buffer, pH 7, stable for several months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-diaminopurine + 5-phospho-alpha-D-ribose 1-diphosphate | specific for adenine or 2,6-diaminopurine | Escherichia coli | 2,6-diaminopurine ribotide + diphosphate | - |
? | |
| 5-amino-4-imidazolecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate | no activity | Escherichia coli | 5-amino-4-imidazolecarboxamide ribotide + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | specific for adenine or 2,6-diamino-purine | Escherichia coli | AMP + diphosphate | 5'-AMP | ? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | enzyme mediates the translocation of adenine into the cell as AMP | Escherichia coli | AMP + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | necessary for appropriate regulation of purine de novo biosynthesis | Escherichia coli | AMP + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, stable | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.33 | - |
adenine | cosubstrate 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Escherichia coli |
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