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Literature summary for 2.4.2.6 extracted from

  • Fernandez-Lucas, J.; Fresco-Taboada, A.; Acebal, C.; de la Mata, I.; Arroyo, M.
    Enzymatic synthesis of nucleoside analogues using immobilized 2-deoxyribosyltransferase from Lactobacillus reuteri (2011), Appl. Microbiol. Biotechnol., 91, 317-327.
    View publication on PubMed
Search for more literature for 2.4.2.6

Application

Application Comment Organism
synthesis covalent attachment of recombinant Lactobacillus reuteri 2'-deoxyribosyltransferase to Sepabeads EC-EP303. The immobilized enzyme retains 50% of its maximal activity after 17.9 h at 60°C, and 96% activity is observed after storage at 40°C for 110 h. Immobilized enzyme can be recycled for 26 consecutive batch reactions in the synthesis of 2,6-diaminopurine-2'-deoxyriboside with negligible loss of catalytic activity and can be employed in the enzymatic synthesis of different natural and therapeutic nucleosides such as 5-ethyl-2'-deoxyuridine and 5-trifluorothymidine Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri A5VK58
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + 5-chlorouracil
-
 Limosilactobacillus reuteri 2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
 ?
2'-deoxyadenosine + cytosine
-
 Limosilactobacillus reuteri 2'-deoxycytidine + adenine
-
 ?
2'-deoxyinosine + uracil
-
 Limosilactobacillus reuteri 2'-deoxyuridine + hypoxanthine
-
 ?
2'-deoxyuridine + 2,6-diaminopurine
-
 Limosilactobacillus reuteri 2,6-diaminopurine 2-deoxy-D-riboside + uracil
-
 ?
2'-deoxyuridine + 5-azacytosine
-
 Limosilactobacillus reuteri 5-aza-2'-deoxycytidine + uracil
-
 ?
2'-deoxyuridine + 5-ethyluracil
-
 Limosilactobacillus reuteri 5-ethyl-2'-deoxyuridine + uracil
-
 ?
2'-deoxyuridine + 5-fluorocytosine
-
 Limosilactobacillus reuteri 2'-deoxy-5-fluorocytidine + uracil
-
 ?
2'-deoxyuridine + 5-methylcytosine
-
 Limosilactobacillus reuteri 5-methyl-2'-deoxycytidine + uracil
-
 ?
2'-deoxyuridine + 6-mercaptopurine
-
 Limosilactobacillus reuteri 6-mercaptopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
 ?
2'-deoxyuridine + adenine
-
 Limosilactobacillus reuteri 2'-deoxyadenosine + uracil
-
 ?
2'-deoxyuridine + benzimidazole
-
 Limosilactobacillus reuteri benzimidazole 2-deoxy-D-ribose 5-phosphate + uracil
-
 ?
2'-deoxyuridine + cytosine
-
 Limosilactobacillus reuteri 2'-deoxycytidine + uracil
-
 ?
2'-deoxyuridine + hypoxanthine
-
 Limosilactobacillus reuteri 2'-deoxyinosine + uracil
-
 ?
thymidine + adenine
-
 Limosilactobacillus reuteri 2'-deoxyadenosine + thymine
-
 ?

Synonyms

Synonyms Comment Organism
NDT4
-
 Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Limosilactobacillus reuteri