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Literature summary for 2.4.2.6 extracted from

  • Kaminski, P.A.; Dacher, P.; Dugue, L.; Pochet, S.
    In vivo reshaping the catalytic site of nucleoside 2-deoxyribosyltransferase for dideoxy- and didehydronucleosides via a single amino acid substitution (2008), J. Biol. Chem., 283, 20053-20059.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A15C kcat is diminished as compared to wild-type Limosilactobacillus fermentum
A15S activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type Limosilactobacillus fermentum
A15T mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type Limosilactobacillus fermentum
A15V can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type Limosilactobacillus fermentum
G9C can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type Lactobacillus leichmannii
G9S mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type Lactobacillus leichmannii
G9T Ser remains a better substituent for Gly-9 than Thr, kcat is diminished as compared to wild-type Lactobacillus leichmannii
G9W can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type Lactobacillus leichmannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
2'-deoxycytidine wild-type Lactobacillus leichmannii
0.3
-
2'-deoxycytidine wild-type Limosilactobacillus fermentum
0.3
-
2'-deoxycytidine mutant G9S Lactobacillus leichmannii
0.38
-
2',3'-dideoxycytidine wild-type Limosilactobacillus fermentum
0.39
-
2'-deoxycytidine mutant A15T Limosilactobacillus fermentum
0.8
-
2',3'-dideoxycytidine mutant A15T Limosilactobacillus fermentum
0.92
-
2',3'-dideoxycytidine mutant G9S Lactobacillus leichmannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18080
-
native enzyme, mass spectrometry Lactobacillus leichmannii
18110
-
mutant, mass spectrometry Lactobacillus leichmannii
18900
-
native enzyme, mass spectrometry Limosilactobacillus fermentum
18930
-
mutant, mass spectrometry Limosilactobacillus fermentum

Organism

Organism UniProt Comment Textmining
Lactobacillus leichmannii Q9RSV5
-
-
Limosilactobacillus fermentum Q6YNI5
-
-
Limosilactobacillus fermentum PAK9 Q6YNI5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Limosilactobacillus fermentum
-
Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
wild-type, with 2',3'-dideoxyguanosine and thymine as substrates Limosilactobacillus fermentum
0.01
-
wild-type, with 2',3'-dideoxyguanosine and thymine as substrates Lactobacillus leichmannii
0.01
-
wild-type, with 2',3'-dideoxyinosine and adenine as substrates Limosilactobacillus fermentum
0.01
-
wild-type, with 2',3'-dideoxyinosine and adenine as substrates Lactobacillus leichmannii
0.01
-
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Lactobacillus leichmannii
0.01
-
wild-type, with 2'-deoxycytidine and hypoxanthine as substrates Lactobacillus leichmannii
0.017
-
wild-type, with 2',3'-dideoxythymidine and adenine as substrates Lactobacillus leichmannii
0.02
-
mutant G9S, with 2'-deoxyinosine and adenine as substrates Limosilactobacillus fermentum
0.03
-
wild-type, with 2',3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
0.036
-
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
0.04
-
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates Lactobacillus leichmannii
0.04
-
wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates Lactobacillus leichmannii
0.05
-
wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
0.056
-
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates Lactobacillus leichmannii
0.06
-
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates Limosilactobacillus fermentum
0.06
-
wild-type, with 2'-deoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
0.19
-
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates Limosilactobacillus fermentum
0.2
-
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Lactobacillus leichmannii
0.25
-
mutant G9S, with 3'-dideoxythymidine and adenine as substrates Lactobacillus leichmannii
0.4
-
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates Lactobacillus leichmannii
0.47
-
mutant G9S, with 2'-deoxyinosine and adenine as substrates Lactobacillus leichmannii
0.5
-
mutant G9S, with 3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
0.57
-
mutant G9S, with 2'-deoxyguanosine and thymine Limosilactobacillus fermentum
0.6
-
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates Limosilactobacillus fermentum
0.8
-
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates Lactobacillus leichmannii
1.04
-
mutant G9S, with 2'-deoxythymidine and adenine as substrates Lactobacillus leichmannii
1.08
-
wild-type, with 2'-deoxyinosine and adenine as substrates Limosilactobacillus fermentum
1.1
-
mutant G9S, with 2'-deoxyguanosine and thymine Lactobacillus leichmannii
1.25
-
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates Lactobacillus leichmannii
1.29
-
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates Limosilactobacillus fermentum
1.3
-
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates Lactobacillus leichmannii
1.6
-
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates Limosilactobacillus fermentum
1.9
-
mutant G9S, with 2'-deoxythymidine and adenine as substrates Limosilactobacillus fermentum
3.1
-
mutant G9S, with 2'-deoxyuridine and cytosine as substrates Lactobacillus leichmannii
3.6
-
wild-type, with 2'-deoxyinosine and adenine as substrates Lactobacillus leichmannii
4.95
-
wild-type, with 2'-deoxyguanosine and thymine Limosilactobacillus fermentum
5.2
-
wild-type, with 2'-deoxyguanosine and thymine Lactobacillus leichmannii
6
-
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates Lactobacillus leichmannii
6.8
-
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
6.9
-
wild-type, with 2',3'-dideoxycytidine and adenine as substrates Limosilactobacillus fermentum
7.1
-
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
9.28
-
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
9.3
-
mutant G9S, with 2'-deoxycytidine and adenine as substrates Limosilactobacillus fermentum
11.9
-
wild-type, with 2'-deoxythymidine and adenine as substrates Lactobacillus leichmannii
12.7
-
mutant G9S, with 2'-deoxyuridine and cytosine as substrates Limosilactobacillus fermentum
20.1
-
wild-type, with 2'-deoxythymidine and adenine as substrates Limosilactobacillus fermentum
27.1
-
mutant G9S, with 2'-deoxycytidine and adenine as substrates Lactobacillus leichmannii
29
-
wild-type, with 2',3'-dideoxycytidine and adenine as substrates Lactobacillus leichmannii
40.2
-
wild-type, with 2'-deoxyuridine and cytosine as substrates Limosilactobacillus fermentum
42.5
-
wild-type, with 2'-deoxyuridine and cytosine as substrates Lactobacillus leichmannii
83.7
-
wild-type, with 2'-deoxycytidine and adenine as substrates Limosilactobacillus fermentum
173
-
wild-type, with 2'-deoxycytidine and adenine as substrates Lactobacillus leichmannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxycytidine + adenine
-
Limosilactobacillus fermentum 2',3'-dideoxyadenosine + cytosine
-
?
2',3'-dideoxycytidine + adenine
-
Lactobacillus leichmannii 2',3'-dideoxyadenosine + cytosine
-
?
2',3'-dideoxycytidine + adenine
-
Limosilactobacillus fermentum PAK9 2',3'-dideoxyadenosine + cytosine
-
?
2',3'-dideoxycytidine + hypoxanthine
-
Limosilactobacillus fermentum 2',3'-dideoxyinosine + cytosine
-
?
2',3'-dideoxycytidine + hypoxanthine
-
Lactobacillus leichmannii 2',3'-dideoxyinosine + cytosine
-
?
2',3'-dideoxycytidine + hypoxanthine
-
Limosilactobacillus fermentum PAK9 2',3'-dideoxyinosine + cytosine
-
?
2',3'-dideoxyguanosine + thymine
-
Limosilactobacillus fermentum 2',3'-dideoxythymidine + guanine
-
?
2',3'-dideoxyguanosine + thymine
-
Lactobacillus leichmannii 2',3'-dideoxythymidine + guanine
-
?
2',3'-dideoxyguanosine + thymine
-
Limosilactobacillus fermentum PAK9 2',3'-dideoxythymidine + guanine
-
?
2',3'-dideoxyinosine + adenine
-
Limosilactobacillus fermentum 2',3'-dideoxyadenosine + hypoxanthine
-
?
2',3'-dideoxyinosine + adenine
-
Lactobacillus leichmannii 2',3'-dideoxyadenosine + hypoxanthine
-
?
2',3'-dideoxyinosine + adenine
-
Limosilactobacillus fermentum PAK9 2',3'-dideoxyadenosine + hypoxanthine
-
?
2',3'-dideoxythymidine + adenine
-
Limosilactobacillus fermentum 2',3'-dideoxyadenosine + thymine
-
?
2',3'-dideoxythymidine + adenine
-
Lactobacillus leichmannii 2',3'-dideoxyadenosine + thymine
-
?
2',3'-dideoxythymidine + adenine
-
Limosilactobacillus fermentum PAK9 2',3'-dideoxyadenosine + thymine
-
?
2',3'-dideoxyuridine + cytosine
-
Limosilactobacillus fermentum 2',3'-dideoxycytidine + uracil
-
?
2',3'-dideoxyuridine + cytosine
-
Lactobacillus leichmannii 2',3'-dideoxycytidine + uracil
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
-
Limosilactobacillus fermentum 2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
-
Lactobacillus leichmannii 2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
?
2'-deoxycytidine + adenine
-
Limosilactobacillus fermentum 2'-deoxyadenosine + cytosine
-
?
2'-deoxycytidine + adenine
-
Lactobacillus leichmannii 2'-deoxyadenosine + cytosine
-
?
2'-deoxycytidine + hypoxanthine
-
Limosilactobacillus fermentum 2'-deoxyinosine + cytosine
-
?
2'-deoxycytidine + hypoxanthine
-
Lactobacillus leichmannii 2'-deoxyinosine + cytosine
-
?
2'-deoxyguanosine + thymine
-
Limosilactobacillus fermentum 2'-deoxythymidine + guanine
-
?
2'-deoxyguanosine + thymine
-
Lactobacillus leichmannii 2'-deoxythymidine + guanine
-
?
2'-deoxyinosine + adenine
-
Limosilactobacillus fermentum 2'-deoxyadenosine + hypoxanthine
-
?
2'-deoxyinosine + adenine
-
Lactobacillus leichmannii 2'-deoxyadenosine + hypoxanthine
-
?
2'-deoxythymidine + adenine
-
Limosilactobacillus fermentum 2'-deoxyadenosine + thymine
-
?
2'-deoxythymidine + adenine
-
Lactobacillus leichmannii 2'-deoxyadenosine + thymine
-
?
2'-deoxyuridine + cytosine
-
Limosilactobacillus fermentum 2'-deoxycytidine + uracil
-
?
2'-deoxyuridine + cytosine
-
Lactobacillus leichmannii 2'-deoxycytidine + uracil
-
?

Synonyms

Synonyms Comment Organism
NTD
-
Limosilactobacillus fermentum
NTD
-
Lactobacillus leichmannii
nucleoside 2'-deoxyribosyltransferase
-
Limosilactobacillus fermentum
nucleoside 2'-deoxyribosyltransferase
-
Lactobacillus leichmannii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.047
-
2',3'-dideoxycytidine wild-type Limosilactobacillus fermentum
2.54
-
2',3'-dideoxycytidine mutant G9S Lactobacillus leichmannii
5.36
-
2'-deoxycytidine mutant A15T Limosilactobacillus fermentum
10.4
-
2',3'-dideoxycytidine mutant A15T Limosilactobacillus fermentum
12.4
-
2'-deoxycytidine mutant G9S Lactobacillus leichmannii
45.1
-
2'-deoxycytidine wild-type Limosilactobacillus fermentum
62.2
-
2'-deoxycytidine wild-type Lactobacillus leichmannii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.12
-
2',3'-dideoxycytidine wild-type Limosilactobacillus fermentum
9.1
-
2',3'-dideoxycytidine mutant G9S Lactobacillus leichmannii
13
-
2',3'-dideoxycytidine mutant A15T Limosilactobacillus fermentum
14
-
2'-deoxycytidine mutant A15T Limosilactobacillus fermentum
41
-
2'-deoxycytidine mutant G9S Lactobacillus leichmannii
150
-
2'-deoxycytidine wild-type Limosilactobacillus fermentum
250
-
2'-deoxycytidine wild-type Lactobacillus leichmannii