Protein Variants | Comment | Organism |
---|---|---|
A15C | kcat is diminished as compared to wild-type | Limosilactobacillus fermentum |
A15S | activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type | Limosilactobacillus fermentum |
A15T | mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type | Limosilactobacillus fermentum |
A15V | can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type | Limosilactobacillus fermentum |
G9C | can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type | Lactobacillus leichmannii |
G9S | mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type | Lactobacillus leichmannii |
G9T | Ser remains a better substituent for Gly-9 than Thr, kcat is diminished as compared to wild-type | Lactobacillus leichmannii |
G9W | can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type | Lactobacillus leichmannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
2'-deoxycytidine | wild-type | Lactobacillus leichmannii | |
0.3 | - |
2'-deoxycytidine | wild-type | Limosilactobacillus fermentum | |
0.3 | - |
2'-deoxycytidine | mutant G9S | Lactobacillus leichmannii | |
0.38 | - |
2',3'-dideoxycytidine | wild-type | Limosilactobacillus fermentum | |
0.39 | - |
2'-deoxycytidine | mutant A15T | Limosilactobacillus fermentum | |
0.8 | - |
2',3'-dideoxycytidine | mutant A15T | Limosilactobacillus fermentum | |
0.92 | - |
2',3'-dideoxycytidine | mutant G9S | Lactobacillus leichmannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18080 | - |
native enzyme, mass spectrometry | Lactobacillus leichmannii |
18110 | - |
mutant, mass spectrometry | Lactobacillus leichmannii |
18900 | - |
native enzyme, mass spectrometry | Limosilactobacillus fermentum |
18930 | - |
mutant, mass spectrometry | Limosilactobacillus fermentum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus leichmannii | Q9RSV5 | - |
- |
Limosilactobacillus fermentum | Q6YNI5 | - |
- |
Limosilactobacillus fermentum PAK9 | Q6YNI5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Limosilactobacillus fermentum |
- |
Lactobacillus leichmannii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
wild-type, with 2',3'-dideoxyguanosine and thymine as substrates | Limosilactobacillus fermentum |
0.01 | - |
wild-type, with 2',3'-dideoxyguanosine and thymine as substrates | Lactobacillus leichmannii |
0.01 | - |
wild-type, with 2',3'-dideoxyinosine and adenine as substrates | Limosilactobacillus fermentum |
0.01 | - |
wild-type, with 2',3'-dideoxyinosine and adenine as substrates | Lactobacillus leichmannii |
0.01 | - |
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates | Lactobacillus leichmannii |
0.01 | - |
wild-type, with 2'-deoxycytidine and hypoxanthine as substrates | Lactobacillus leichmannii |
0.017 | - |
wild-type, with 2',3'-dideoxythymidine and adenine as substrates | Lactobacillus leichmannii |
0.02 | - |
mutant G9S, with 2'-deoxyinosine and adenine as substrates | Limosilactobacillus fermentum |
0.03 | - |
wild-type, with 2',3'-dideoxythymidine and adenine as substrates | Limosilactobacillus fermentum |
0.036 | - |
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates | Limosilactobacillus fermentum |
0.04 | - |
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates | Lactobacillus leichmannii |
0.04 | - |
wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates | Lactobacillus leichmannii |
0.05 | - |
wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates | Limosilactobacillus fermentum |
0.056 | - |
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates | Lactobacillus leichmannii |
0.06 | - |
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates | Limosilactobacillus fermentum |
0.06 | - |
wild-type, with 2'-deoxycytidine and hypoxanthine as substrates | Limosilactobacillus fermentum |
0.19 | - |
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates | Limosilactobacillus fermentum |
0.2 | - |
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates | Lactobacillus leichmannii |
0.25 | - |
mutant G9S, with 3'-dideoxythymidine and adenine as substrates | Lactobacillus leichmannii |
0.4 | - |
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates | Lactobacillus leichmannii |
0.47 | - |
mutant G9S, with 2'-deoxyinosine and adenine as substrates | Lactobacillus leichmannii |
0.5 | - |
mutant G9S, with 3'-dideoxythymidine and adenine as substrates | Limosilactobacillus fermentum |
0.57 | - |
mutant G9S, with 2'-deoxyguanosine and thymine | Limosilactobacillus fermentum |
0.6 | - |
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates | Limosilactobacillus fermentum |
0.8 | - |
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates | Lactobacillus leichmannii |
1.04 | - |
mutant G9S, with 2'-deoxythymidine and adenine as substrates | Lactobacillus leichmannii |
1.08 | - |
wild-type, with 2'-deoxyinosine and adenine as substrates | Limosilactobacillus fermentum |
1.1 | - |
mutant G9S, with 2'-deoxyguanosine and thymine | Lactobacillus leichmannii |
1.25 | - |
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates | Lactobacillus leichmannii |
1.29 | - |
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates | Limosilactobacillus fermentum |
1.3 | - |
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates | Lactobacillus leichmannii |
1.6 | - |
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates | Limosilactobacillus fermentum |
1.9 | - |
mutant G9S, with 2'-deoxythymidine and adenine as substrates | Limosilactobacillus fermentum |
3.1 | - |
mutant G9S, with 2'-deoxyuridine and cytosine as substrates | Lactobacillus leichmannii |
3.6 | - |
wild-type, with 2'-deoxyinosine and adenine as substrates | Lactobacillus leichmannii |
4.95 | - |
wild-type, with 2'-deoxyguanosine and thymine | Limosilactobacillus fermentum |
5.2 | - |
wild-type, with 2'-deoxyguanosine and thymine | Lactobacillus leichmannii |
6 | - |
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates | Lactobacillus leichmannii |
6.8 | - |
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates | Limosilactobacillus fermentum |
6.9 | - |
wild-type, with 2',3'-dideoxycytidine and adenine as substrates | Limosilactobacillus fermentum |
7.1 | - |
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates | Limosilactobacillus fermentum |
9.28 | - |
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates | Limosilactobacillus fermentum |
9.3 | - |
mutant G9S, with 2'-deoxycytidine and adenine as substrates | Limosilactobacillus fermentum |
11.9 | - |
wild-type, with 2'-deoxythymidine and adenine as substrates | Lactobacillus leichmannii |
12.7 | - |
mutant G9S, with 2'-deoxyuridine and cytosine as substrates | Limosilactobacillus fermentum |
20.1 | - |
wild-type, with 2'-deoxythymidine and adenine as substrates | Limosilactobacillus fermentum |
27.1 | - |
mutant G9S, with 2'-deoxycytidine and adenine as substrates | Lactobacillus leichmannii |
29 | - |
wild-type, with 2',3'-dideoxycytidine and adenine as substrates | Lactobacillus leichmannii |
40.2 | - |
wild-type, with 2'-deoxyuridine and cytosine as substrates | Limosilactobacillus fermentum |
42.5 | - |
wild-type, with 2'-deoxyuridine and cytosine as substrates | Lactobacillus leichmannii |
83.7 | - |
wild-type, with 2'-deoxycytidine and adenine as substrates | Limosilactobacillus fermentum |
173 | - |
wild-type, with 2'-deoxycytidine and adenine as substrates | Lactobacillus leichmannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-dideoxycytidine + adenine | - |
Limosilactobacillus fermentum | 2',3'-dideoxyadenosine + cytosine | - |
? | |
2',3'-dideoxycytidine + adenine | - |
Lactobacillus leichmannii | 2',3'-dideoxyadenosine + cytosine | - |
? | |
2',3'-dideoxycytidine + adenine | - |
Limosilactobacillus fermentum PAK9 | 2',3'-dideoxyadenosine + cytosine | - |
? | |
2',3'-dideoxycytidine + hypoxanthine | - |
Limosilactobacillus fermentum | 2',3'-dideoxyinosine + cytosine | - |
? | |
2',3'-dideoxycytidine + hypoxanthine | - |
Lactobacillus leichmannii | 2',3'-dideoxyinosine + cytosine | - |
? | |
2',3'-dideoxycytidine + hypoxanthine | - |
Limosilactobacillus fermentum PAK9 | 2',3'-dideoxyinosine + cytosine | - |
? | |
2',3'-dideoxyguanosine + thymine | - |
Limosilactobacillus fermentum | 2',3'-dideoxythymidine + guanine | - |
? | |
2',3'-dideoxyguanosine + thymine | - |
Lactobacillus leichmannii | 2',3'-dideoxythymidine + guanine | - |
? | |
2',3'-dideoxyguanosine + thymine | - |
Limosilactobacillus fermentum PAK9 | 2',3'-dideoxythymidine + guanine | - |
? | |
2',3'-dideoxyinosine + adenine | - |
Limosilactobacillus fermentum | 2',3'-dideoxyadenosine + hypoxanthine | - |
? | |
2',3'-dideoxyinosine + adenine | - |
Lactobacillus leichmannii | 2',3'-dideoxyadenosine + hypoxanthine | - |
? | |
2',3'-dideoxyinosine + adenine | - |
Limosilactobacillus fermentum PAK9 | 2',3'-dideoxyadenosine + hypoxanthine | - |
? | |
2',3'-dideoxythymidine + adenine | - |
Limosilactobacillus fermentum | 2',3'-dideoxyadenosine + thymine | - |
? | |
2',3'-dideoxythymidine + adenine | - |
Lactobacillus leichmannii | 2',3'-dideoxyadenosine + thymine | - |
? | |
2',3'-dideoxythymidine + adenine | - |
Limosilactobacillus fermentum PAK9 | 2',3'-dideoxyadenosine + thymine | - |
? | |
2',3'-dideoxyuridine + cytosine | - |
Limosilactobacillus fermentum | 2',3'-dideoxycytidine + uracil | - |
? | |
2',3'-dideoxyuridine + cytosine | - |
Lactobacillus leichmannii | 2',3'-dideoxycytidine + uracil | - |
? | |
2',3'didehydro-2',3'-dideoxythymidine + adenine | - |
Limosilactobacillus fermentum | 2',3'didehydro-2',3'-dideoxyadenosine + thymine | - |
? | |
2',3'didehydro-2',3'-dideoxythymidine + adenine | - |
Lactobacillus leichmannii | 2',3'didehydro-2',3'-dideoxyadenosine + thymine | - |
? | |
2'-deoxycytidine + adenine | - |
Limosilactobacillus fermentum | 2'-deoxyadenosine + cytosine | - |
? | |
2'-deoxycytidine + adenine | - |
Lactobacillus leichmannii | 2'-deoxyadenosine + cytosine | - |
? | |
2'-deoxycytidine + hypoxanthine | - |
Limosilactobacillus fermentum | 2'-deoxyinosine + cytosine | - |
? | |
2'-deoxycytidine + hypoxanthine | - |
Lactobacillus leichmannii | 2'-deoxyinosine + cytosine | - |
? | |
2'-deoxyguanosine + thymine | - |
Limosilactobacillus fermentum | 2'-deoxythymidine + guanine | - |
? | |
2'-deoxyguanosine + thymine | - |
Lactobacillus leichmannii | 2'-deoxythymidine + guanine | - |
? | |
2'-deoxyinosine + adenine | - |
Limosilactobacillus fermentum | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2'-deoxyinosine + adenine | - |
Lactobacillus leichmannii | 2'-deoxyadenosine + hypoxanthine | - |
? | |
2'-deoxythymidine + adenine | - |
Limosilactobacillus fermentum | 2'-deoxyadenosine + thymine | - |
? | |
2'-deoxythymidine + adenine | - |
Lactobacillus leichmannii | 2'-deoxyadenosine + thymine | - |
? | |
2'-deoxyuridine + cytosine | - |
Limosilactobacillus fermentum | 2'-deoxycytidine + uracil | - |
? | |
2'-deoxyuridine + cytosine | - |
Lactobacillus leichmannii | 2'-deoxycytidine + uracil | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NTD | - |
Limosilactobacillus fermentum |
NTD | - |
Lactobacillus leichmannii |
nucleoside 2'-deoxyribosyltransferase | - |
Limosilactobacillus fermentum |
nucleoside 2'-deoxyribosyltransferase | - |
Lactobacillus leichmannii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
2',3'-dideoxycytidine | wild-type | Limosilactobacillus fermentum | |
2.54 | - |
2',3'-dideoxycytidine | mutant G9S | Lactobacillus leichmannii | |
5.36 | - |
2'-deoxycytidine | mutant A15T | Limosilactobacillus fermentum | |
10.4 | - |
2',3'-dideoxycytidine | mutant A15T | Limosilactobacillus fermentum | |
12.4 | - |
2'-deoxycytidine | mutant G9S | Lactobacillus leichmannii | |
45.1 | - |
2'-deoxycytidine | wild-type | Limosilactobacillus fermentum | |
62.2 | - |
2'-deoxycytidine | wild-type | Lactobacillus leichmannii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2',3'-dideoxycytidine | wild-type | Limosilactobacillus fermentum | |
9.1 | - |
2',3'-dideoxycytidine | mutant G9S | Lactobacillus leichmannii | |
13 | - |
2',3'-dideoxycytidine | mutant A15T | Limosilactobacillus fermentum | |
14 | - |
2'-deoxycytidine | mutant A15T | Limosilactobacillus fermentum | |
41 | - |
2'-deoxycytidine | mutant G9S | Lactobacillus leichmannii | |
150 | - |
2'-deoxycytidine | wild-type | Limosilactobacillus fermentum | |
250 | - |
2'-deoxycytidine | wild-type | Lactobacillus leichmannii |