General Stability | Organism |
---|---|
2 mM dithiothreitol and 10% (v/v) glycerol stabilize the enzyme activity during long-term storage and subsequent purification steps | Methanosarcina thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(methylamino)benzoic acid | 5 mM, complete loss of activity | Methanosarcina thermophila | |
4-aminothiobenzoate | 5 mM, 24% loss of activity | Methanosarcina thermophila | |
4-Bromobenzoate | 5 mM, 60% loss of activity | Methanosarcina thermophila | |
4-hydroxybenzoate | 5 mM, 88% loss of activity | Methanosarcina thermophila | |
4-Methoxybenzoate | 5 mM, 55% loss of activity | Methanosarcina thermophila | |
benzoate | 5 mM, 31% loss of activity | Methanosarcina thermophila | |
diphosphate | 5 mM, 84% inhibition | Methanosarcina thermophila | |
additional information | no inhibition by carbonyl reagents, 4-aminobenzoic acid methyl ester, aniline, 4-aminobenzamide | Methanosarcina thermophila | |
phosphate | 10 mM, 37% inhibition | Methanosarcina thermophila | |
pyridoxal 5'-phosphate | 2.5 mM, 21% inhibition | Methanosarcina thermophila | |
ribose 5-phosphate | 10 mM, 20% inhibition | Methanosarcina thermophila | |
Sodium cyanoborohydride | 160 mM, complete inhibition, decreased production of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate and the accumulation of a reduced form of the proposed cyclohexadienimine reaction intermediates | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
4-Aminobenzoate | pH 4.8, 50°C | Methanosarcina thermophila | |
3.6 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 4.8, 50°C | Methanosarcina thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | reaction is dependent on Mg2+ or Mn2+, optimal concentration: 10 mM | Methanosarcina thermophila | |
Mn2+ | reaction is dependent on Mg2+ or Mn2+ | Methanosarcina thermophila | |
additional information | requires no dissociable trace metal ions such as iron or cobalt ion for activity | Methanosarcina thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
1 * 64000, SDS-PAGE | Methanosarcina thermophila |
65000 | - |
gel filtration | Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | Methanosarcina thermophila | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
- |
- |
Oxidation Stability | Organism |
---|---|
relatively stable in air | Methanosarcina thermophila |
Purification (Comment) | Organism |
---|---|
partial | Methanosarcina thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.03 | - |
pH 4.8, 50°C | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria | Methanosarcina thermophila | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? | |
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | sequential pattern of substrate binding. An SN1-like reaction produces oxonium ion character at C-1 of 5-phospho-alpha-D-ribose 1-diphosphate which undergoes an ipso electrophilic aromatic substitution reaction at the carboxylic acid-bound carbon of 4-aminobenzoate. Decarboxylation of the resulting cyclohexadienimine intermediate leads to the formation of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate | Methanosarcina thermophila | 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate | - |
? | |
5-phospho-alpha-D-ribose 1-diphosphate + 4-hydroxybenzoate | sequential pattern of substrate binding | Methanosarcina thermophila | 4-(beta-D-ribofuranosyl)hydroxybenzene 5'-phosphate + CO2 + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, SDS-PAGE | Methanosarcina thermophila |
Synonyms | Comment | Organism |
---|---|---|
beta-RFA-P synthase | - |
Methanosarcina thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Methanosarcina thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Methanosarcina thermophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
the activity of the enzyme pretreated for 30 min at pH 4.0 is 48% of the activity for enzyme treated at either pH 4.8 or 5.9 | Methanosarcina thermophila |
4.8 | - |
the activity of the enzyme pretreated for 30 min at pH 4.0 is 48% of the activity for enzyme treated at either pH 4.8 or 5.9 | Methanosarcina thermophila |
5.9 | - |
the activity of the enzyme pretreated for 30 min at pH 4.0 is 48% of the activity for enzyme treated at either pH 4.8 or 5.9 | Methanosarcina thermophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
4-hydroxybenzoate | pH 4.8, 50°C, Ki(slope) | Methanosarcina thermophila | |
0.59 | - |
4-Bromobenzoate | pH 4.8, 50°C, Ki(slope) | Methanosarcina thermophila |