Literature summary for 2.4.2.45 extracted from

Huang, H.; Berg, S.; Spencer, J.S.; Vereecke, D.; DHaeze, W.; Holsters, M.; McNeil, M.R.
Identification of amino acids and domains required for catalytic activity of DPPR synthase, a cell wall biosynthetic enzyme of Mycobacterium tuberculosis (2008), Microbiology, 154, 736-743.

Search for more literature for 2.4.2.45

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycolicibacterium smegmatis
expressed in Escherichia coli BL21(DE3) cells	Corynebacterium glutamicum
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
A66F	inactive	Mycobacterium tuberculosis
D74A	inactive	Mycobacterium tuberculosis
D77A	inactive	Mycobacterium tuberculosis
D77E	15% activity compared to the wild type enzyme	Mycobacterium tuberculosis
D81A	inactive	Mycobacterium tuberculosis
E195A	17% activity compared to the wild type enzyme	Mycobacterium tuberculosis
F59A	94% activity compared to the wild type enzyme	Mycobacterium tuberculosis
F62A	72% activity compared to the wild type enzyme	Mycobacterium tuberculosis
H84L	15% activity compared to the wild type enzyme	Mycobacterium tuberculosis
N29A	18% activity compared to the wild type enzyme	Mycobacterium tuberculosis
N73A	inactive	Mycobacterium tuberculosis
N73Q	15% activity compared to the wild type enzyme	Mycobacterium tuberculosis
R192A	inactive	Mycobacterium tuberculosis
R201A	1% activity compared to the wild type enzyme	Mycobacterium tuberculosis
R22L	10% activity compared to the wild type enzyme	Mycobacterium tuberculosis
Y70A	inactive	Mycobacterium tuberculosis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Mycolicibacterium smegmatis	5886	-
plasma membrane	-	Corynebacterium glutamicum	5886	-
plasma membrane	-	Mycobacterium tuberculosis	5886	-

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	-	-	-
Mycobacterium tuberculosis	P9WFR5	-	-
Mycolicibacterium smegmatis	-	-	-
no activity in Azorhizobium caulinodans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate	-	Mycolicibacterium smegmatis	trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate	-	?
trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate	-	Corynebacterium glutamicum	trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate	-	?
trans,octacis-decaprenyl phosphate + 5-phospho-alpha-D-ribose 1-diphosphate	-	Mycobacterium tuberculosis	trans,octacis-decaprenylphospho-beta-D-ribofuranose 5-phosphate + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
5-phospho-alpha-D-ribose-1-diphosphate: decaprenyl-phosphate	-	Mycolicibacterium smegmatis
5-phospho-alpha-D-ribose-1-diphosphate: decaprenyl-phosphate	-	Corynebacterium glutamicum
5-phospho-alpha-D-ribose-1-diphosphate: decaprenyl-phosphate	-	Mycobacterium tuberculosis
5-phosphoribosyltransferase	-	Mycolicibacterium smegmatis
5-phosphoribosyltransferase	-	Corynebacterium glutamicum
5-phosphoribosyltransferase	-	Mycobacterium tuberculosis
cg3189	-	Corynebacterium glutamicum
DPPR synthase	-	Mycolicibacterium smegmatis
DPPR synthase	-	Corynebacterium glutamicum
DPPR synthase	-	Mycobacterium tuberculosis
MSMEG 6401	-	Mycolicibacterium smegmatis
Rv3806c	-	Mycobacterium tuberculosis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)