Any feedback?
Literature summary for 2.4.2.4 extracted from
- Optimum induction of recombinant thymidine phosphorylase and its application (2011), Nucleosides Nucleotides Nucleic Acids, 30, 360-368.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | construction of Escherichia coli expression vector pDEOA and use of lactose instead of IPTG to induce expression. The use of lactose at concentrations above 0.5 mmol/l has an induction effect similar to that of IPTG but results in a longer initial induction time and better cell growth. The thymidine phosphorylase induced by lactose is very stable at 50°C. Intact pDEOA cells induced by lactose can be used as a source of thymidine phosphorylase. Under standard reaction conditions, several deoxynucleosides are effectively produced from thymidine | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
