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Literature summary for 2.4.2.4 extracted from

  • Panova, N.G.; Shcheveleva, E.V.; Alexeev, C.S.; Mukhortov, V.G.; Zuev, A.N.; Mikhailov, S.N.; Esipov, R.S.; Chuvikovsky, D.V.; Miroshnikov, A.I.
    Use of 4-thiouridine and 4-thiothymidine in studies on pyrimidine nucleoside phosphorylases (2004), Mol. Biol., 38, 770-776.
No PubMed abstract available
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Inhibitors

Inhibitors Comment Organism Structure
1-(2-hydroxyethoxymethyl)thymine
-
 Escherichia coli
2,2'-O-anhydrouridine
-
 Escherichia coli
5-fluoro-1-(tetrahydrofur-2-yl)uracil
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 4-thiothymidine pH 7.5, 25°C Escherichia coli
0.3
-
 thymidine pH 7.5, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-thiothymidine + phosphate
-
 Escherichia coli 4-thiothymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
thymidine + phosphate
-
 Escherichia coli thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
63
-
 4-thiothymidine pH 7.5, 25°C Escherichia coli
198
-
 thymidine pH 7.5, 25°C Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
 2,2'-O-anhydrouridine pH 7.5, 25°C, activity with 4-thiothymidine Escherichia coli
0.4
-
 5-fluoro-1-(tetrahydrofur-2-yl)uracil pH 7.5, 25°C, activity with thymidine Escherichia coli
0.45
-
 5-fluoro-1-(tetrahydrofur-2-yl)uracil pH 7.5, 25°C, activity with 4-thiothymidine Escherichia coli
0.5
-
 2,2'-O-anhydrouridine pH 7.5, 25°C, activity with thymidine Escherichia coli
1.5
-
 1-(2-hydroxyethoxymethyl)thymine pH 7.5, 25°C, activity with 4-thiothymidine Escherichia coli